Expression and Purification of GFP-E90DOPA by a Biosynthetic System
4:25
Oligomerization of Purified GFP-E90DOPA and SDS-PAGE Analysis
5:47
Conjugation of GFP-E90DOPA with an Alkyne Probe by SPOCQ, Purification of the Labeled GFP
7:10
SDS-PAGE Analysis and Fluorescence Gel Scanning of Labeled GFP
8:21
Results: Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine
10:01
Conclusion
Burada, L-dihydroxyphenylalanine uzarlar basit başlangıç malzemelerden genetik birleşme ve protein konjugasyon için uygulama için bir iletişim kuralı mevcut.