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DOI :
10.3791/58407-v
•
8:36 min
November 1st, 2018
Chapters
0:04
Title
1:06
Synthesis of the DENV 3’UTR RNA Standard
3:15
Processing of Virus Samples for RT-qPCR
4:19
Real-time PCR Analysis
6:01
Results: Real-time Quantitative Polymerase Chain Reaction of Virus RNA
7:58
Conclusion
实时定量聚合酶链反应分析与逆转录酶链反应 (rt-qpcr) 相结合, 已被广泛用于测量 rna 病毒感染水平。在这里, 我们提出了一个直接的 rt-qpcr 检测, 不需要 rna 纯化步骤, 开发的几个 rna 病毒, 包括登革热病毒的定量。
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