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DOI :
10.3791/58421-v
•
10:50 min
November 2nd, 2018
Chapters
0:04
Title
1:27
Preparation and Coating of the Multicompartment Chips
3:57
Seeding Neurons into the Multicompartment Chips
5:03
Fluidic Isolation of the Axonal Compartment within the Chip
5:49
Performing Axotomy Within the Chip
6:36
Fluorescence Immunostaining within the Chip
9:03
Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
10:21
Conclusion
该协议描述了使用塑料芯片培养和分割主要小鼠神经元。这些芯片是预装的, 用户友好的, 并与高分辨率, 活的和荧光成像兼容。该方案描述了如何在这些芯片内对大鼠海马神经元进行平板治疗, 并进行流控分离、轴切和免疫染色。
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