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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

DOI :

10.3791/58433-v

September 28th, 2018

September 28th, 2018

14,097 Views

1Pulmonary Department, The Chaim Sheba Medical Center, 2Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center

Here, we present a protocol for co-immunoprecipitation and an on-bead enzymatic activity assay to simultaneously study the contribution of specific protein domains of plasma membrane receptors to both enzyme recruitment and enzyme activity.

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