JoVE Logo
Faculty Resource Center

Sign In

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes (Video) | JoVE

DOI :

10.3791/58433-v

September 28th, 2018

September 28th, 2018

14,269 Views

1Pulmonary Department, The Chaim Sheba Medical Center, 2Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center

Here, we present a protocol for co-immunoprecipitation and an on-bead enzymatic activity assay to simultaneously study the contribution of specific protein domains of plasma membrane receptors to both enzyme recruitment and enzyme activity.

-- Views

Related Videos

article

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

article

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

article

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

article

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

article

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture (Video) | JoVE

article

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions (Video) | JoVE

article

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens (Video) | JoVE

article

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium (Video) | JoVE

article

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems (Video) | JoVE

article

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved