Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

21.1K Views

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09:42 min

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December 23rd, 2014

DOI :

10.3791/52199-v

December 23rd, 2014

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Mahdieh Tabatabaei Shafiei¹, Catalina M. Carvajal Gonczi¹, Mohammed Samiur Rahman², Ashley East³, Jonathan François³, Peter J. Darlington¹^,³

¹Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ²Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ³Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

Summary

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Keywords PAS Staining

Glycogen

Peripheral Blood Mononuclear Cells

Chapters in this video

0:05

Title

1:34

PBMC Isolation from Whole Blood

5:15

Making the PBMC Slide and Fixing the Samples

6:13

Making the Amylase Solution for Negative Control

7:00

Perform Periodic Acid Schiff (PAS) Staining and Imaging

8:11

Results: PAS-staining on Human PBMCs

9:17

Conclusion

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