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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

DOI :

10.3791/52199-v

December 23rd, 2014

December 23rd, 2014

20,756 Views

1Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 2Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 3Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.

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Keywords PAS Staining

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