JoVE Logo
Faculty Resource Center

Sign In

Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

DOI :

10.3791/58437-v

5:47 min

February 12th, 2019

February 12th, 2019

18,990 Views

1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado

Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.

Tags

3D Lung Tissue Cultures

-- Views

Related Videos

article

A Simple Method of Mouse Lung Intubation

article

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

article

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

article

Quantitative 3D In Silico Modeling (q3DISM) of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease

article

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue

article

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

article

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

article

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

article

Three-Dimensional Reconstruction for the Whole Lung with Early Multiple Pulmonary Nodules

article

Automated Vibratome Sectioning of Agarose-Embedded Lung Tissue for Multiplex Fluorescence Imaging

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved