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Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

DOI :

10.3791/58471-v

5:27 min

January 26th, 2019

January 26th, 2019

22,086 Views

1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Division of Laboratory Animal Resources, University of Kentucky, 3River Hill Ranch

A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.

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Immunization

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