Name: subclavian vein puncture as an alternative method of blood sample collection in rats
Uploaded: 2018-11-18T13:00:00
Description: Watch this Scientific Journal Video about Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats at JoVE.com

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本研究获得湘雅第二医院 (中国长沙) 中南大学动物研究伦理委员会批准。该手稿是根据 arrive (动物研究: 活体实验报告) 指南9编写的。
1. 材料和动物准备
<ol><li>准备所需材料: 胶带、脱毛剂、75% 乙醇、采血管、2.5 毫升注射器与针头连接 (24 g、0.6 mm x25 毫米)、剃须刀、电子秤和仪表 (见材料表)。</li>
	<li>使用 20只 sprague-dawley (sd) 大鼠, 年龄为 8-10...

<ol>
	<li>Parasuraman, S., Raveendran, R., Kesavan, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+sample+collection+in+small+laboratory+animals.">Blood sample collection in small laboratory animals.</a> Journal of Pharmacology, Pharmacotherapeutics. 1 (2), 87-93 (2010).</li><li>Korfmacher, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Utility+of+capillary+microsampling+for+rat+pharmacokinetic+studies:+Comparison+of+tail-vein+bleed+to+jugular+vein+cannula+sampling.">Utility of capillary microsampling for rat pharmacokinetic studies: Comparison of tail-vein bleed to jugular vein cannula sampling.</a> Journal of Pharmacological and Toxicological Methods. 76, 7-14 (2015).</li><li>Feng, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catheterization+of+the+carotid+artery+and+jugular+vein+to+perform+hemodynamic+measures,+infusions+and+blood+sampling+in+a+conscious+rat+model.">Catheterization of the carotid artery and jugular vein to perform hemodynamic measures, infusions and blood sampling in a conscious rat model.</a> Journal of Visualized Experiments. 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J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determining+Glucose+Metabolism+Kinetics+Using+18F-FDG+Micro-PET/CT.">Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT.</a> Journal of Visualized Experiments. (123), (2017).</li><li>Beeton, C., Garcia, A., Chandy, K. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drawing+blood+from+rats+through+the+saphenous+vein+and+by+cardiac+puncture.">Drawing blood from rats through the saphenous vein and by cardiac puncture.</a> Journal of Visualized Experiments. 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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improving+bioscience+research+reporting:+ARRIVE-ing+at+a+solution.">Improving bioscience research reporting: ARRIVE-ing at a solution.</a> Lab Animal. 44 (4), 377-378 (2010).</li><li>National Institute of Health. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Guide for the Care and Use of Laboratory Animals. , (2011).</li><li>Antonelli, D., Feldman, A., Freedberg, N. A., Turgeman, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axillary+vein+puncture+without+contrast+venography+for+pacemaker+and+defibrillator+leads+implantation.">Axillary vein puncture without contrast venography for pacemaker and defibrillator leads implantation.</a> Pacing and Clinical Electrophysiology. 36 (9), 1107-1110 (2013).</li><li>Luis, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+magnetic+resonance+imaging+of+the+thoracic+venous+system+in+rats+with+congenital+diaphragmatic+hernia.">Microscopic magnetic resonance imaging of the thoracic venous system in rats with congenital diaphragmatic hernia.</a> Pediatric Surgery International. 27 (2), 175-180 (2011).</li><li>Tsukamoto, A., Serizawa, K., Sato, R., Yamazaki, J., Inomata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vital+signs+monitoring+during+injectable+and+inhalant+anesthesia+in+mice.">Vital signs monitoring during injectable and inhalant anesthesia in mice.</a> Experimental Animals. 64 (1), 57-64 (2015).</li></ol>

本文介绍了一种从锁骨下静脉抽血的替代方法, 表明该方法是可行的、安全的, 适用于大鼠的采血。该方法来源于11例起搏器铅植入术中锁骨下静脉穿刺.锁骨下静脉位于锁骨的后部和尾端, 是腋窝静脉的延续。它从第一肋骨的外缘延伸到前鳞片肌肉12的内侧边界。从这里, 它加入颈内静脉形成腕头静脉, 并流入超级腔静脉, 就在上星下 (图 8)。...

采血在动物研究中是必不可少的。采血的目标静脉包括眼眶后丛、颈静脉、隐静脉、尾血管和颈动脉1、2、3、4。有时, 血液可以从腹主动脉, 静脉, 甚至心脏5,6,7。在这种情况下, 必须牺牲动物, 不能用于后续观察;因此, 这些方法在日常实验工作中的使用较少。与上述方法相比, 从大鼠尾静脉采集血液很受欢迎, 压力较小 8。
然而, 从尾部静脉采集血液有时受到不令人满意的成功率的限制。有时, 用这种方法也很难获得足够的血量。由于锁骨下静脉足够大, 位于锁骨骨的正下方, 如果常规采血方法不成功, 锁骨下静脉穿刺可以成为采血的另一种方法。本文介绍了一种大鼠锁骨下静脉穿刺采血的方法。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>rats</td>
			<td>Hunan SJA Laboratory Animal Co., Ltd (Changsha, Hunan Province, China)&#160;</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>stastical software</td>
			<td>SPSS Statistics 17</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>epilating agent</td>
			<td>France Yi Sha Cosmetics Co.,Ltd(Guangzhou, Guangdong Province ,China)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>2.5 mL syringe</td>
			<td>Shandong Weigao Group Medical Polymer Co.,Ltd(Weihai,Shandong Province ,China)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>hair shaver</td>
			<td>Shanghai FLYCO Electric Co., Ltd(Shanghai,China)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>adhesive tape</td>
			<td>3M Deutschland GmbH(EdisonstraBe 6,59157 Kamen, Germany)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Pentobarbital sodium</td>
			<td>Merck</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>75% ethanol</td>
			<td>Department of Pharmacy,The Second Xiangya Hospital of Central South Univesity</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>blood collection tube</td>
			<td>Hubei Jinxing Technology&#38;Development Co.,Ltd (Wuhan Hubei Province,China) (2ml)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>electronic scale</td>
			<td>Dongguan Shengheng Electronics Co.,Ltd (Dongguan,Guangdng Province,China)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>canvas gloves</td>
			<td>for anethesia</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>hepain</td>
			<td>Nanjing Xinbai Pharmaceutical&#160; Co.,Limited (Nanjing, Jiangsu Province, China) (2mL, 12500 IU)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>physical saline</td>
			<td>Hunan Kelun Pharmaceutical Co., Ltd(Yueyang ,Hunan Province,China) (100ml)</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

subclavian vein puncture as an alternative method of blood sample collection in rats

在动物实验中, 有足够的血量的采血是必不可少的。与其他更具攻击性的方法 (如后眼眶神经丛样本采集) 相比, 从大鼠尾脉采集血液很受欢迎, 压力也较小。然而, 这种采血方法有时受到不令人满意的成功率的限制。在这里, 我们介绍了一种方法, 血液收集通过锁骨下静脉穿刺。锁骨下静脉位于锁骨下, 这个静脉足够大, 以满足血液采集的体积要求。结果表明, 该方法是安全的, 适用于所需血量的采血取样。在大鼠尾静脉采血失败的情况下, 通过锁骨下静脉穿刺采血可作为一种替代采血方法。

这里共使用了 20只 sd 大鼠 (雄性 n示 10, 雌性？ 10)。血液收集是由熟练的操作人员进行的, 他们在大鼠的锁骨下静脉进行了20多个血液取样, 初学者操作人员则没有在大鼠或其他动物身上进行锁骨下静脉穿刺的经验。在操作之前, 初学者至少观察了由熟练操作人员完成的3个过程。
血液取样成功于17只大鼠通过右锁骨下静脉通道。?...

Watch this Scientific Journal Video about Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats at JoVE.com

Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats

在这里, 我们提出了一个从大鼠锁骨下静脉采集血液样本的方案。

锁骨下静脉穿刺作为大鼠采血的一种替代方法

Blood collection with enough blood volume is essential in animal experiments. Blood collection from the tail vein of rats is popular and less stressful ...

亚克拉维安静脉穿刺作为大鼠血液样本采集的替代方法。全身麻醉后，将大鼠放在苏平位置，用75%乙醇对下层空间进行消毒。在穿刺前识别优越的尾骨。找到穿刺点，在同一侧的锁骨外三分之一区域。针的方向被定位到优越的胸骨，应该几乎平行的锁骨，只是后到它。采集血液后，按穿刺位点一到两分钟，止血。在动物实验中，总是需要收集足够量的血液。从大鼠尾静脉采集血液比一些攻击性方法（如复古轨道丛样本采集）更受欢迎，压力也更小。但是，此方法受成功率限制。在这里，我们介绍我们的血液收集方法与所需的血液量从亚克拉维安静脉。结果表明，该方法安全实用，在尾静脉血液采样失败的情况下可考虑。首先，准备胶带、脱毛剂、75%乙醇、血液收集管、二点五毫升注射器、剃毛剂、电子秤、肝素和生理盐水。家鼠在标准条件下，可免费获得食物和饮用水，被关在530平方厘米的笼子里，有木屑床上用品。穿刺前通过内刺注射诱导所有动物的全身麻醉。将大鼠放在超级位置，将后肢固定在舒适的位置，并固定与身体的上肢平行，就在树干旁边。清洁隔光空间的两侧，以去除毛皮和任何可见的污垢。用 75% 乙醇擦拭颈部和胸皮，用纱布保持区域清洁干燥。亚克拉维安静脉的两侧都适合穿刺，在这里，我们选择右侧进行穿刺。用操作员的左拇指确定右锁骨的位置和方向，用食指轻轻定位上司的胸骨。使用每毫升 10 个单位肝素溶液冲洗我们的注射器。找到穿刺点，指向右锁骨外三分之一区域五毫米的考达尔。轻轻地将注射器移入穿刺点的皮肤。一旦针头进入下层皮肤，通过操作员的右手施加主动压力。针的方向定位在上司胸叶，应该几乎平行的锁骨，只是后背。通常，通过在此方向插入针头约两毫米，可以到达亚克拉维安静脉。一旦针头进入静脉，血液就会在活动压力下进入注射器。然后保持活动压力，直到抽出适当的血液量。采集血液后，取出穿刺针，然后按穿刺位点一到两分钟以止血。注意：压力必须非常温和，以防止窒息。将血液样本与EDTA一起转移到抗凝真空管中，并在1600G下离心10分钟收集血浆。吸气的超级支架重新清洁管和储存在零下18摄氏度。为了比较熟练操作者与初学者的成功率，随机将20只SD大鼠分成两组。结果表明，通过右亚克拉维脉，技术组的9只大鼠和初学者组的8只大鼠分别成功。然后从剩下的三只老鼠身上采集血液，成功地通过左侧。这两个群体的成功率没有显著差异。这两组的时间路线略有不同。手术期间或手术后无严重并发症，如肺炎、血红素和出血。所有动物在血液取样后都幸存了下来。为了证明学习穿刺程序的困难，我们进一步比较了熟练组和初学者组之间的取款数量。如表二所示，熟练群体的平均取款数量略低于初学者，但没有统计学意义。结果表明，亚克拉维安静脉穿刺在大鼠身上采集血液是安全可行的。这种方法是有价值的，特别是在需要大量血液的时候。如果常规血液采样失败，此方法可能是最佳替代方案。

Research

JoVE Journal

Medicine

Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections

舌下免疫治疗作为一种替代起到保护作用对急性呼吸道感染

Sublingual route has been widely used to deliver small molecules into the bloodstream and to modulate the immune response at different sites. It has been ...

科研

医学

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System

无麻醉大鼠尾静脉血采集系统的研究

Blood can be collected from rats in a number of sampling locations. For instance, the tail vein is a superior location for blood sampling. However, the ...

Routine Screening Method for Microparticles in Platelet Transfusions

血小板输血中微粒的常规筛选方法

Platelet inventory management based on screening microparticle content in platelet concentrates is a new quality improvement initiative for hospital blood ...

Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis

穿刺诱导的虹膜新生血管虹膜虹膜小鼠模型的建立

We describe a model of puncture-induced iris neovascularization as a general model for noninvasive evaluation of angiogenesis. The model is also relevant ...

Blood Collection Through Subclavian Vein Puncture in Mice

小鼠锁骨下静脉穿刺采血

The mouse is the foremost mammalian model for studying human disease and human health. However, blood sample collection from mice is challenging in ...

The Rigid Tube as an Alternative in Controlling the Problematic Airway

刚性管作为控制问题气道的替代方案

The unexpected problematic airway represents a large proportion of anesthesia-related morbidity and mortality. The retromolar or paraglossal approach is ...

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

建立大鼠永久性颈静脉插管的显微外科技能，用于口服药物的连续血液采样

Blood sampling in small laboratory animals is necessary for pharmaceutical lead optimization but can cause great harm and stress to experimental animals, ...

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to ...

Comparative Study of Blood Collection Techniques in Rats

Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model

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改良尾静脉和静脉穿刺用于大鼠模型中的血液采样

Blood samples are required in most experimental animal designs to assess various hematological parameters. This paper presents two procedures for blood ...