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DOI :
10.3791/58503-v
June 28th, 2019
Chapters
0:04
Title
0:43
Femoral Vein and Artery Catheterization
4:46
Tracer Administration and Collection of Blood Samples
6:07
Analysis of Plasma Samples to Determine Concentrations of Leucine and L-[1-14C]leucine
7:07
Quantitative Autoradiography
7:58
Image Analysis and Computation of Regional Rates of Cerebral Protein Synthesis (rCPS)
9:02
Results: Effects of Anisomycin on Rates of Cerebral Protein Synthesis
9:57
Conclusion
タンパク質合成は、細胞にとって重要な生物学的プロセスです。脳では、適応的な変化のために必要とされる。無傷の脳におけるタンパク質合成率の測定には、慎重な方法論的考慮が必要である。ここでは、生体内における脳タンパク質合成の局所速度を決定するためのL-[1-14 C]-ロイシン定量的自動放射線法を紹介する。
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