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DOI :
10.3791/58537-v
•
10:28 min
November 2nd, 2018
Chapters
0:04
Title
0:24
NMR Experimental Setup
4:25
NMR Data Processing
5:47
NMR Data Analysis
8:13
Results: Vimentin 99-249 (VimRod) Binds Wildtype Envoplakin Plakin Repeat Domain (E-PRD) but not Mutant R1914E E-PRD
9:54
Conclusion
在这里, 我们提出了一个协议, 生产和纯化的蛋白质标记与稳定同位素, 以及随后的表征蛋白质相互作用使用核磁共振 (nmr) 光谱和微尺度热像仪 (mst) 实验。
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