Name: continuous noninvasive measuring of crayfish cardiac and behavioral activities
Uploaded: 2019-02-06T14:00:00
Description: Watch this Scientific Journal Video about Continuous Noninvasive Measuring of Crayfish Cardiac and Behavioral Activities at JoVE.com

这项研究得到了捷克共和国教育、青年和体育部 "cenakva" 项目 "cenakva" 项目的支持。CZ.1.05/2.1.00/01.0024 和 "cenakva ii" 否。根据国家可持续发展方案 i, 由捷克布杰约维采的南波希米亚大学赠款机构 (012/2016/z) 和捷克共和国赠款机构 (16-06498s 号) 提交

1. 小龙虾选择
<ol><li>为了成功地将目前的方法应用于小龙虾, 请选择具有足够甲壳大小 (甲壳长度至少为30毫米) 的相应成人标本用于传感器附件, 对其是否有疾病进行目视检查, 并进行检查它是否在触摸时将两个切菜都抬起。上述参数表明小龙虾健康状况合格。 请注意:如果在试验中预计将使用几条小龙虾, 并暴露在相同的条件下, 应根据几个参数形成实验组: (一) 重量和长度相似;(ii...

<ol>
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V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Total+protein+in+hemolymph+of+crawfish+Pontastacus+leptodactylus+as+a+parameter+of+the+functional+state+of+animals+and+a+biomarker+of+quality+of+habitat.">Total protein in hemolymph of crawfish Pontastacus leptodactylus as a parameter of the functional state of animals and a biomarker of quality of habitat.</a> Journal of Evolutionary Biochemistry and Physiology. 47 (2), 160-167 (2011).</li><li>Pautsina, A., Kuklina, I., &#352;tys, D., C&#237;sa&#345;, P., Koz&#225;k, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+crayfish+cardiac+activity+monitoring+system.">Noninvasive crayfish cardiac activity monitoring system.</a> Limnology and Oceanography: Methods. 12 (10), 670-679 (2014).</li><li>C&#237;sa&#345;, P., Saberioon, M., Koz&#225;k, P., Pautsina, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fully+contactless+system+for+crayfish+heartbeat+monitoring:+Undisturbed+crayfish+as+bio-indicator.">Fully contactless system for crayfish heartbeat monitoring: Undisturbed crayfish as bio-indicator.</a> Sensors and Actuators B: Chemical. 255, 29-34 (2018).</li></ol>

人们普遍认为, 测量某些生理参数 (如心脏或通气率或两者兼而有之) 是记录小龙虾反应的更可靠的方法, 而不是对并不总是发生的行为反应的评价立刻11。然而, 评估小龙虾对环境变化的真正反应的最有效方法显然是心脏活动和行为记录的结合, 因为这样就可以看到小龙虾心跳的原因这些变化是否因周围环境的化学变化或运动的开始而发生。在水质监测过程中, 至关重要的是要消除...

水生生物的应用主题, 既是各种实验室调查的示范生物 1、2,也是监测工业和自然环境水质的工具 3、4, 似乎是很好的研究。然而, 这一主题仍然是人类值得注意的问题, 无论他们是属于科学界还是属于其他职业。尽管存在一些先进的方法来监测某些参数 (所谓的 "生物标志物")5,6, 7,8,选择指标由三个简单因素组成: (一) 简单性, (二) 可靠性, (三) 一般可用性。
小龙虾作为淡水动物的重要代表, 之所以与众不同, 是因为它在世界范围内很普遍, 而且在大多数情况下,9 有足够大和坚硬的甲壳, 适合操作。这种甲壳类动物属于较高的无脊椎动物群体, 它们提供了重要的生理系统和各自器官的充分发展, 同时保持了一个相对简单的组织10。
方法在对小龙虾的生物学和行为参数范围进行评估的基础上, 对一般生物监测和小龙虾研究的发展作出了重大贡献。目前可用的小龙虾心率测量侵入性方法大多是基于心电图记录, 需要复杂而精确的外科手术11,12,13;这样的操作可能会对小龙虾造成巨大的压力, 并可能需要长时间的适应。另外, 不知道小龙虾能携带这样的电极多久, 也不知道它在携带这种附件的同时是否会成功蜕皮。所述的无创方法是基于平面记录的, 这由于硬件复杂性而变得复杂, 需要一个信号滤波的调理电路14和一个放大或精确而昂贵的光学元件15 ,16。
在这项研究中, 我们描述了一种有助于现有结果的方法, 并为改进当前的小龙虾心率测量程序提供了新的替代方法。在这些优点中, 有 (i) 快速和无创的依恋, 不需要长时间的生理适应;(ii) 小龙虾在从成型到蜕花的几个月内携带传感器的能力;(iii) 能够监测实时心脏和行为活动以及评估同时从多条小龙虾获得的数据的软件;(iv) 制造价格低、简单。我们描述的生物监测系统允许根据小龙虾的伦理生理特性的变化对小龙虾心脏和运动活动进行无创和持续监测。该系统可轻松应用于小龙虾心脏生理和伦理的实验室检查, 以及控制水处理和供水设施水质的工业实施。

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			<td height="21" style="height:21px;width:216px;">IR LED diode</td>
			<td style="width:180px;">KINGBRIGHT ELECTRONIC</td>
			<td style="width:155px;">KP-3216F3C</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Phototransistor</td>
			<td style="width:180px;">EVERLIGHT</td>
			<td>ELPT15-21C</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Resistor</td>
			<td style="width:180px;">ROYAL OHM</td>
			<td style="width:155px;">0805S8J0201T5E</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Resistor</td>
			<td style="width:180px;">ROYAL OHM</td>
			<td style="width:155px;">0805S8F2200T5E</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Capacitor</td>
			<td style="width:180px;">KEMET</td>
			<td style="width:155px;">C0805C334K5RACTU</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Cable</td>
			<td style="width:180px;">TECHNOKABEL</td>
			<td style="width:155px;">FTP KAT.5E 4X2X0,14C</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Connector</td>
			<td style="width:180px;">HARTING</td>
			<td style="width:155px;">21348100380005</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Connector</td>
			<td style="width:180px;">HARTING</td>
			<td style="width:155px;">21348000380005</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dielectric gel</td>
			<td style="width:180px;">KRAYDEN</td>
			<td style="width:155px;">Sylgard 535</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Analogue-to-digital convertor</td>
			<td style="width:180px;">TEDIA</td>
			<td style="width:155px;">UDAQ-1416CA</td>
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			<td height="21" style="height:21px;width:216px;">Glue</td>
			<td style="width:180px;">KUPSITO.SK</td>
			<td>7338723044</td>
			<td></td>
		</tr>
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			<td height="21" style="height:21px;width:216px;">Kinect video camera</td>
			<td style="width:180px;">ABCSTORE.CZ</td>
			<td style="width:155px;">GT3-00002</td>
			<td></td>
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			<td height="126" style="height:126px;width:216px;">Analysis software</td>
			<td style="width:180px;">University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Institute of Complex Systems</td>
			<td style="width:155px;"></td>
			<td style="width:167px;">Link to the software: www.frov.jcu.cz/crayfishmonitoring 
			User name: frov 
			Password: CF2018</td>
		</tr>
	</tbody>
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continuous noninvasive measuring of crayfish cardiac and behavioral activities

小龙虾是一种重要的水生生物, 既是无脊椎动物行为和生理研究的实用生物模型, 也是水质的有用生物指标。尽管小龙虾不能直接指定导致水质恶化的物质, 但它们可以立即 (在几秒钟内) 通过心脏和行为活动的剧烈变化警告人类水质恶化。
在本研究中, 我们提出了一种非侵入性方法, 由于一个模型结合了简单性和可靠性, 可以在各种条件下实现。
这种将生物生物应用到环境评价过程中的方法, 为警告和防止环境中的急性水恶化提供了可靠和及时的警报。因此, 对这种基于小龙虾生理和伦理参数记录的无创系统进行了研究, 以检测水生环境中的变化。该系统现已应用于当地一家啤酒厂, 用于控制饮料生产用水的质量, 但可用于任何水处理和供水设施, 用于持续、实时的水质评价和定期实验室研究小龙虾心脏生理和行为。

因此, 我们获得了小龙虾心脏和行为活动的组合, 记录并保存在 txt 格式的文件中 (图 3)。除了实验小龙虾的数量、日期和采样率外, 该文件还包括三列: (1) 以 hh:mm:ss 格式连续的时间;(2) 心率以每分钟节拍自动计算;(3) 登记为没有 (0) 或存在任何运动的运动。当小龙虾处于非活动状态时, 将零分配给负责移动的细胞, 当它移动时, 第一个就出现在各自的细?...

Watch this Scientific Journal Video about Continuous Noninvasive Measuring of Crayfish Cardiac and Behavioral Activities at JoVE.com

Continuous Noninvasive Measuring of Crayfish Cardiac and Behavioral Activities

小龙虾心脏的连续无创测量与行为活动

本文介绍了一种用于小龙虾心脏和运动活动连续记录和分析的无创生物监测系统。该系统由近红外光学传感器、视频跟踪模块和评估小龙虾心跳的软件组成, 该软件反映了小龙虾的生理状态, 并描述了小龙虾在心跳波动期间的行为特征。

A crayfish is a pivotal aquatic organism that serves both as a practical biological model for behavioral and physiological studies of invertebrates and as ...

该方法有助于回答环境和生态研究领域涉及心脏运动活动与运动活动在各种急性和长期压力条件下的关系。这种技术的主要优点是，小龙虾不需要复杂的先前操作或长期适应，并能够轴承传感器之间的方法几个月。该技术的影响扩展到病因学、生理学、生态毒理学和工业应用，因为小龙虾辅助非侵入性生物监测可以在实验室、水处理和供应设施中实施。虽然这种方法可以提供对小龙虾心脏、生理、行为和水质控制的内部洞察，但它有可能应用于其他大型淡水和海洋甲壳类动物。该方法的视觉演示对于理解使用近红外光进行小龙虾心脏评估的非侵入性传感器与基于图像处理的运动检测模块之间的联系至关重要。在开始手术的前几天，选择至少30毫米的甲壳大小的成人标本。并检查每个标本是否没有疾病，以及他们是否解除两个切利触摸时。然后让健康的小龙虾适应一罐已解决的自来水，直到手术的一天。一旦传感器准备好，打开计算机上的传感器软件，并输入要固定在传感器并在屏幕上显示的小龙虾数量。设置要可视化的小龙虾心率数。用纸巾擦干第一只动物的后甲。用纸巾包裹小龙虾的切利和腹部，以避免损坏，并消除对动物的额外压力。并附加审查器的后甲壳在一个位置，有利于最大的心脏信号振幅。一只手拿着传感器小龙虾，用另一只手在传感器上的四根辅助导线上添加一滴新鲜混合的环氧树脂胶。然后让胶水干燥，而不移动传感器至少五分钟。当胶水不再粘在触摸上时，将未包装的小龙虾和传感器放入一个没有水的盒子里几分钟，直到胶水完全干燥。在将小龙虾放回水箱之前，将头孢菌管浸入水箱水中几秒钟，以排出刺中积聚的空气。将小龙虾留在水箱中约一小时，以清除多余的化学物质。然后，根据观测到的生理指数，将小龙虾释放到实验水箱中一至两周的适应时间，并在适当的实验条件下。一旦小龙虾被放置在实验罐中，启动软件。摄像机将自动打开。然后选择运动检测选项，并在屏幕上定位每个水箱，开始跟踪行为，将小龙虾的行为与心脏活动记录联系起来。记录的小龙虾心脏和行为活动可以保存在一个TXT格式的文件。在分析中包括小龙虾的运动至关重要，因为这种活动可能导致心率的变化。例如，在这个实验开始十秒钟后，食物气味通过近体泵被传递到水箱中。在14秒内，小龙虾识别了刺激，由于所谓的定向反应，其心率略有下降。20秒后，心率增加，导致心脏间隔减少。在26秒时，小龙虾向刺激源移动，食物气味引起的生理刺激和运动启动导致心率显著增加。在37秒时，有证据表明小龙虾突然运动，在刺激反应期间，这些小龙虾的突然运动可能大大促进了心率的增长。事实上，不安的小龙虾通常表现出心率的增加，这通常与偶尔的运动有关。然而，即使是一动不动的小龙虾也可以表现出高心率，也表明有明显的压力。未受干扰小龙虾的心率的特点是心跳曲线的单调振幅和每个心脏峰值之间大致相等的心脏间隔。在尝试此过程时，重要的是要记住，快速和彻底的传感器附件将减轻实验动物的压力，从而获得精确的生理特征。更先进的监测方法包括使用完全非接触式小龙虾监测，允许仅使用近红外传感器和敏感摄像头的组合确定心跳频率。这种方法开发后，为无限制小龙虾和其他大型水生无脊椎动物的行为、生理学、繁殖和雄激素领域的研究人员铺平了道路，以探索环境和人为对生物二代生物的影响。对于非侵入性生物监测，在捷克当地品种中具有非常实用的应用，作为实时水质早期监测系统。同时对小龙虾生态生理特征的动态进行连续评估，对水状态的稳定性进行评价。

Research

JoVE Journal

Behavior

Behavioral Phenotyping of Murine Disease Models with the Integrated Behavioral Station (INBEST)

Behavioral Phenotyping of Murine Disease Models with the Integrated Behavioral Station INBEST

小鼠疾病模型的集成行为车站行为表型（INBEST）

Due to rapid advances in genetic engineering, small rodents have become the preferred subjects in many disciplines of biomedical research. In studies of ...

科研

行为学

Measuring Neural and Behavioral Activity During Ongoing Computerized Social Interactions: An Examination of Event-Related Brain Potentials

测量神经和行为活动期间持续计算机化的社会互动：对事件相关电位的考察

Social exclusion is a complex social phenomenon with powerful negative consequences. Given the impact of social exclusion on mental and emotional health, ...

A Novel Experimental and Analytical Approach to the Multimodal Neural Decoding of Intent During Social Interaction in Freely-behaving Human Infants

在自由，表现人类婴儿一种新的实验和分析方法意向的多模态神经解码期间社会互动

Understanding typical and atypical development remains one of the fundamental questions in developmental human neuroscience. Traditionally, experimental ...

The Rodent Psychomotor Vigilance Test (rPVT): A Method for Assessing Neurobehavioral Performance in Rats and Mice

The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

鼠类精神运动警觉测试（rPVT）：一个在大鼠和小鼠的神经行为评估性能的方法

The human Psychomotor Vigilance Test (PVT) is a widely used procedure for measuring changes in fatigue and sustained attention. The present article ...

Using Wavelet Entropy to Demonstrate how Mindfulness Practice Increases Coordination between Irregular Cerebral and Cardiac Activities

使用小波熵来证明正念练习如何增加不规则脑与心脏活动之间的协调

In both the East and West, traditional teachings say that the mind and heart are somehow closely correlated, especially during spiritual practice. One ...

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

鱼类生殖生态学研究的基本方法

Captive-rearing observations are valuable for revealing aspects of fish behavior and ecology when continuous field investigations are impossible. Here, a ...

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

用于体内的无线双向接口记录和刺激自由行为大鼠的神经活动

In vivo electrophysiology is a powerful technique to investigate the relationship between brain activity and behavior at a millisecond and micrometer ...

Noninvasive, High-throughput Determination of Sleep Duration in Rodents

无创高通量测定啮齿类动物睡眠时间

Traditionally, sleep is monitored by an electroencephalogram (EEG). EEG studies in rodents require surgical implantation of the electrodes followed by a ...

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in  Free Moving Rodents

基于自动 T 迷宫的自由移动啮齿动物延迟和努力决策分析装置及协议

Many neurological and psychiatric patients demonstrate difficulties and/or deficits in decision making. Rodent models are helpful to produce a deeper ...

Noninvasive, In-pen Approach Test for Laboratory-housed Pigs

实验室安置猪的非侵入性、笔内方法测试

Traumatic brain injury (TBI) incidences have increased in both civilian and military populations, and many researchers are adopting a porcine model for ...