Name: a pleural effusion model in rats by intratracheal instillation of polyacrylate/nanosilica
Uploaded: 2019-04-12T13:00:00
Description: Watch this Scientific Journal Video about A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica at JoVE.com

本报告的研究和制作由国家自然科学基金资助 (赠款817373、81773373和赠款 81773373)。我们感谢北京朝阳医院急诊科金燕博士和潘玉杰医生以及北京朝阳医院超声科的曲鹏医生对视频制作的帮助。

这项研究遵循了首都医科大学 (中国北京) 关于实验动物护理和使用的指导方针。所有程序均由中国首都医科大学动物伦理委员会批准。
1. 实验制剂
注: 在给药前一周 (环境条件: ligeny:12 h\ 12h, 温度 22±2°c, 湿度 50±10%)。
<ol><li>使用新鲜的10毫升 paumnsi 悬浮液 (纳米直径: 20±5 nm 通过原位乳液聚合) 在正常盐水中稀释, 浓度分别为3.125、6.25 和 12.5 mgml, 分别为 1...

<ol>
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超声检查是确定肺部疾病最方便的工具, 因为它对胸腔11中的游离液体具有出色的敏感性.这是因为超声可以立即检测到肺12中空气和液体的声阻抗的对比.此外, 在小型动物的模型中, 超声比 CT 更灵活。然而, 肺中的空气反映了声波, 阻碍了观察纳米颗粒注入后的肺内变化。因此, 我们结合胸部 CT 扫描和肺超声检查, 以调查肺内的变化和胸膜液。
<p class="jove_content...

胸腔积液是肺部疾病的一种非常常见的临床表现, 其原因多种多样。建立一个有用的动物胸腔积液模型对研究这些肺部疾病、两层胸膜层的作用、胸腔积液的机制及其治疗具有重要意义。然而, 一些报道的胸腔积液模型主要集中在恶性胸腔积液或生物因素上, 而不是环境中的纳米颗粒 1,2。本文介绍了一种简单、安全、有效的胸腔积液新模型。
随着纳米技术的发展和纳米产品的广泛使用, 人们对纳米材料对环境和人类健康的潜在危害表示担忧.纳米材料引入了风险因素, 并可能导致工作场所或环境污染带来新的危害。体外和体内研究表明, 纳米材料可导致多器官损伤的肺, 心脏, 肝脏, 肾脏, 和神经系统, 以及生殖和免疫系统的5,6。此外, 一些研究报告说, 纳米材料的具体毒性是由于其独特的物理化学特性3,4,7。
我们报告说, 一组职业接触纳米材料的工人临床上出现胸膜和心包积液、肺纤维化和肉芽肿 8,9.在这些患者的胸腔积液中分离出二氧化硅纳米颗粒9。为了复制和验证吸入纳米颗粒在人体中引起的胸腔积液, 我们通过大鼠的呼吸道注入聚丙烯/纳米二氧化硅 (pac7月 nnosi), 模拟了人体的呼吸。环境, 并发现气管内灌注 papacsi 可导致大鼠胸腔积液。本文介绍了如何通过气管内注入 pansi 制作大鼠胸腔积液, 以及如何分离胸腔积液中的纳米颗粒。该模型可用于进一步研究纳米毒理学和胸腔积液疾病。

<table border="0" cellpadding="0" cellspacing="0" style="width:843px;" width="843">
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	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:227px;">Acuson S2000 Color Doppler ultrasound system</td>
			<td style="width:196px;">Siemens Medical Solutions, Mountain View ,CA</td>
			<td style="width:160px;"></td>
			<td style="width:260px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:227px;">&#160;Polyacrylate/nanosilica</td>
			<td style="width:196px;">Fudan University,Shanghai, China</td>
			<td style="width:160px;"></td>
			<td>made by order with nanosilica(20&#177;5)nm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">10% chloral hydrate</td>
			<td style="width:196px;">Beijing Chemical Works</td>
			<td style="width:160px;">302-17-0</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:227px;">Transmission electron microscope&#160;</td>
			<td style="width:196px;">JEM-1400Plus&#65292;JEOL Ltd., Japan.</td>
			<td style="width:160px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:227px;">Light speed 16 spiral computed tomography</td>
			<td style="width:196px;">GE Healthcare, US</td>
			<td style="width:160px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:227px;">Specific pathogen-free Wistar</td>
			<td style="width:196px;">Animal Center of Lianhelihua (Beijing, China)</td>
			<td style="width:160px;"></td>
			<td>Wistar rats</td>
		</tr>
	</tbody>
</table>

a pleural effusion model in rats by intratracheal instillation of polyacrylate/nanosilica

胸腔积液是许多肺部疾病的临床流行发现。建立一个有用的动物胸腔积液模型对研究这些肺部疾病具有重要意义。以往的胸腔积液模型更关注的是生物因素, 而不是环境中的纳米颗粒。本文介绍了用气管内注入聚丙烯酸/纳米菌的方法制作大鼠胸腔积液的模型, 以及一种在胸腔积液中分离纳米颗粒的方法。通过气管内注入浓度为3.125、6.25 和 12.5 mg/kg * ml 的聚丙烯酸盐, 在第3天出现的大鼠胸腔积液, 在6.25 天和 12.5 mg kg * 组中达到7-10 天的高峰, 然后在第14天慢慢减少和消失。当聚丙烯/纳米二氧化硅浓度增加时, 胸腔积液产生的速度越来越快。经超声检查或 CT 胸部扫描检测这种胸膜液, 并经大鼠解剖证实。透射电镜观察大鼠胸腔积液中的二氧化硅纳米颗粒。这些结果表明, 接触聚丙烯/纳米二氧化硅导致胸腔积液的诱导, 这与我们上一次在人类身上的报告是一致的。此外, 该模型还有利于进一步研究纳米毒理学和胸腔积液疾病。

使用胸腔超声, 我们在第一天没有发现所有组的胸腔积液。然而, 在第3天, 胸腔积液出现在6.25 和 12.5 mgkg ·Ml 组。积液主要呈右肋脑角, 心包积液仅表现为 12.5 mg/kg mL 组。此外, 第7天, 在 6.25 mg/kg ·ml 组 (图 1) 中检测到胸腔积液 (视频 1) 和心包积液 (视频2)。胸腔积液在7-10 内增长缓慢, 然后逐渐减少。第14天, 没有发现胸腔积液, ...

Watch this Scientific Journal Video about A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica at JoVE.com

A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica

本文提出了用气管内注入聚丙烯酸/纳米二氧化硅建立大鼠胸腔积液模型的方案。

聚丙烯酸/纳米水的气管内灌注积液模型

Pleural effusion is a prevalent clinical finding of many pulmonary diseases. Having a useful animal pleural effusion model is very important to study ...

该方法可对纳米材料的毒性进行洞察，并可用于肺毒性研究。该技术用于研究多丙烯酸酯/纳米硅诱导胸膜灌注的影响。在开始灌输之前，在普通盐水中对新鲜、10毫升、多丙烯酸酯/纳米硅悬浮液进行20至30分钟的声波化，然后进行10分钟的涡旋，然后将悬浮液稀释至每毫升浓度3.125、6.25和12.5毫克。接下来，确认对麻醉鼠脚趾捏的缺乏反应，用一根尼龙绳将动物的门牙固定到板上。使用钳子和正面让，轻轻地张开嘴，以可视化球状的裂缝，并插入一个细管到双边支气管。然后，将0.5毫升的多丙烯酸酯/纳米硅颗粒通过管子注入肺部，将大鼠放在监测的末端位置，直至完全恢复。在灌输后的第一天、第三天、第七天和第14天，使用电动剃须刀将头发从每只麻醉大鼠的胸部和上腹部去除，并放置大鼠在超声波系统的安装板上，并采用线性阵列传感器在上部位置。将超声波凝胶涂抹在暴露的皮肤上，将传感器放在成本空间和子成本区域，以检测胸膜液体。为了准确检测输液，选择左右两个位置进行超声波检查。在第七天，在每毫升灌输组每公斤6.25毫克中检测到胸膜输液和心包外输液。在第7天和14日，CT胸部扫描每公斤12.5毫克每毫升灌输的动物是异常的钝后食觉角度，暗示少量的胸膜灌注。胸膜输液样品的透射电子显微镜显示纳米硅纳米粒子单独和集群与在准备的悬浮液内观测到的纳米粒子的形态一致。最重要的是记住想象球状的裂缝，并在双边支气管中插入细管。纳米粒子也可以通过吸入或静脉注射进行，因为两种方法在以前的研究中都进行了。这项技术对研究胸膜输液的发展是有用的。

Research

JoVE Journal

Medicine

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model

一种新的手术方法气管内注射生物活性剂在胎鼠模型

Prenatal pulmonary delivery of cells, genes or pharmacologic agents could provide the basis for new therapeutic strategies for a variety of genetic and ...

科研

医学

A Contusion Model of Severe Spinal Cord Injury in Rats

挫伤模型脊髓严重损伤的保护作用

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

Intubation-mediated Intratracheal IMIT Instillation: A Noninvasive, Lung-specific Delivery System

插管介导气管内（IMIT）灌输：一种无创，龙专用输送系统

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences ...

Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction

Retroductal颌下腺灌输和本地化分割照射在涎腺功能减退症大鼠模型

Normal tissues that lie within the portals of radiation are inadvertently damaged. Salivary glands are often injured during head and neck radiotherapy. ...

A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats

心脏重构通过大鼠腹主动脉缩窄的模型

Heart failure is one of the leading causes of death worldwide. It is a complex clinical syndromethat includes fatigue, dyspnea, exercise intolerance, and ...

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Circumlimbal 缝合法诱导大鼠和小鼠青光眼模型的建立

The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known ...

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

通过直接内切内脂多糖灌输诱导小鼠急性肺损伤

Airway administration of lipopolysaccharide (LPS) is a common way to study pulmonary inflammation and acute lung injury (ALI) in small animal models. ...

Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats

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The current animal models of osteoarthritis (OA) can be divided into spontaneous models and induced models, both of which aim to simulate the ...

Intratracheal Instillation of Stem Cells in Term Neonatal Rats

期新生儿大鼠干细胞的内切内培养

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Treatment of neonatal rodent with drugs instilled directly into the trachea could serve as a valuable tool to study the impact of a locally administered ...