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DOI :
10.3791/58568-v
November 30th, 2018
Chapters
0:04
Title
1:01
Ligation-independent Cloning (LIC)
2:38
E. coli Transformation and Protein Expression
4:58
Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification
6:12
Protein Crystallization, Crystal Mounting, and Structure Determination
9:56
Results: Representative N-terminal Domain (NTD) Crystal Structure Determination
10:53
Conclusion
この記事で私たちはコナガ (コナガ) からリアノジン受容体の N 末端ドメインの蛋白質の発現、精製、結晶化、構造決定のプロトコルを説明します。
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