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DOI :
10.3791/58589-v
•
5:30 min
January 23rd, 2019
Chapters
0:04
Title
0:43
Main Culture Cultivation and Harvesting
3:30
Results: Representative De-Repressed Protein Expression Analysis
4:32
Conclusion
该方法首次介绍了在毕赤酵母 (komagataella phaffii)高效诱导无甲醇重组蛋白生产的可靠实验程序, 并采用了碳源调节启动子 p dc和 pdf 在小规模试验中, 在可在线监测培养参数的同时, 可采用恒定的甘油饲料。
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