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DOI :
10.3791/58596-v
•
10:55 min
November 10th, 2018
Chapters
0:00
Title
1:06
Sample Preparation
1:43
RNA Extraction
6:08
RNA Concentration Measurement
6:35
cDNA Synthesis
7:09
Real-time Polymerase Chain Reaction (qPCR)
9:27
Representative Results
10:22
Conclusion
该协议使用 rt-pcr 方法诊断罗非鱼组织中的罗非鱼病毒 (tilv)。整个方法描述了从组织解剖到总 rna 提取, 然后是 cdna 合成和检测 tilv 的常规 pcr 或定量 pcr 使用 dsdna 结合染料。
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