Name: development and angiographic use of the rabbit vx2 model for liver cancer
Uploaded: 2019-01-07T14:00:00
Description: Watch this Scientific Journal Video about Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer at JoVE.com

我们要感谢伊利诺伊大学芝加哥生物资源实验室的兽医工作人员。

以下协议遵循伊利诺伊大学芝加哥分校规定的所有要求和准则。它在执行前经过了当地动物护理和使用机构委员会的审查和批准。
1. vx2 hind 肢体肿瘤的发展
<ol><li>从国家癌症研究所癌症治疗诊断和治疗肿瘤细胞系存储库中获得 vx2 肿瘤细胞系。 注意: 此时, 订单目录可在以下链接中找到: https://dtp.cancer.gov/repositories/。</li>
	<li>准备注射用细胞悬浮液, 将冷冻的 vx2 样品和甲基...

<ol>
	<li>Rous, P., Beard, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Progression+To+Carcinoma+of+Virus-Induced+Rabbit+Papillomas+(Shope).">The Progression To Carcinoma of Virus-Induced Rabbit Papillomas (Shope).</a> The Journal of Experimental Medicine. 62 (4), 523-548 (1935).</li><li>Kidd, J. G., Rous, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+transplantable+rabbit+carcinoma+originating+in+a+virus-induced+papilloma+and+containing+the+virus+in+masked+or+altered+form.">A transplantable rabbit carcinoma originating in a virus-induced papilloma and containing the virus in masked or altered form.</a> The Journal of Experimental Medicine. 71 (6), 813-838 (1940).</li><li>Galasko, C. S. B., Muckle, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intrasarcolemmal+proliferation+of+the+vx2+carcinoma.">Intrasarcolemmal proliferation of the vx2 carcinoma.</a> British Journal of Cancer. 29 (1), 59-65 (1974).</li><li>Maruyama, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sonographic+shift+of+hypervascular+liver+tumor+on+blood+pool+harmonic+images+with+definity:+Time-related+changes+of+contrast-enhanced+appearance+in+rabbit+VX2+tumor+under+extra-low+acoustic+power.">Sonographic shift of hypervascular liver tumor on blood pool harmonic images with definity: Time-related changes of contrast-enhanced appearance in rabbit VX2 tumor under extra-low acoustic power.</a> European Journal of Radiology. 56 (1), 60-65 (2005).</li><li>Horkan, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiofrequency+Ablation:+Effect+of+Pharmacologic+Modulation+of+Hepatic+and+Renal+Blood+Flow+on+Coagulation+Diameter+in+a+VX2+Tumor+Model.">Radiofrequency Ablation: Effect of Pharmacologic Modulation of Hepatic and Renal Blood Flow on Coagulation Diameter in a VX2 Tumor Model.</a> Journal of Vascular and Interventional Radiology. 15 (3), 269-274 (2004).</li><li>Bimonte, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+VX2+para-renal+carcinoma+in+rabbits:+generation+of+animal+model+for+loco-regional+treatments+of+solid+tumors.">Induction of VX2 para-renal carcinoma in rabbits: generation of animal model for loco-regional treatments of solid tumors.</a> Infectious Agents and Cancer. 11 (1), 1-8 (2016).</li><li>Goldberg, S. N., Gazelle, G. S., Compton, C. C., Mueller, P. R., McLoud, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radio-frequency+tissue+ablation+of+VX2+tumor+nodules+in+the+rabbit+lung.">Radio-frequency tissue ablation of VX2 tumor nodules in the rabbit lung.</a> Academic Radiology. 3 (11), 929-935 (1996).</li><li>Rhee, T. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rabbit+VX2+Tumors+as+an+Animal+Model+of+Uterine+Fibroids+and+for+Uterine+Artery+Embolization.">Rabbit VX2 Tumors as an Animal Model of Uterine Fibroids and for Uterine Artery Embolization.</a> Journal of Vascular and Interventional Radiology. 18 (3), 411-418 (2007).</li><li>Parvinian, A., Casadaban, L. C., Gaba, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development,+growth,+propagation,+and+angiographic+utilization+of+the+rabbit+VX2+model+of+liver+cancer:+A+pictorial+primer+and+&amp;#34;how+to&amp;#34;+guide.">Development, growth, propagation, and angiographic utilization of the rabbit VX2 model of liver cancer: A pictorial primer and &amp;#34;how to&amp;#34; guide.</a> Diagnostic and Interventional Radiology. 20 (4), 335-340 (2014).</li><li>Xia, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-arterial+interleukin-12+gene+delivery+combined+with+chemoembolization:+Anti-tumor+effect+in+a+rabbit+hepatocellular+carcinoma+(HCC)+model.">Intra-arterial interleukin-12 gene delivery combined with chemoembolization: Anti-tumor effect in a rabbit hepatocellular carcinoma (HCC) model.</a> Acta Radiologica. 54 (6), 684-689 (2013).</li><li>Gaba, R. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ethiodized+oil+uptake+does+not+predict+doxorubicin+drug+delivery+after+chemoembolization+in+VX2+liver+tumors.">Ethiodized oil uptake does not predict doxorubicin drug delivery after chemoembolization in VX2 liver tumors.</a> Journal of Vascular and Interventional Radiology. 23 (2), 265-273 (2012).</li><li>Choi, Y. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+Intraarterial+Therapy+for+Liver+Cancer+Using+Ethylbromopyruvate+Dissolved+in+an+Iodized+Oil.">Novel Intraarterial Therapy for Liver Cancer Using Ethylbromopyruvate Dissolved in an Iodized Oil.</a> Academic Radiology. 18 (4), 471-478 (2011).</li><li>Ma, H. L., Xu, Y. F., Qi, X. R., Maitani, Y., Nagai, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Superparamagnetic+iron+oxide+nanoparticles+stabilized+by+alginate:+Pharmacokinetics,+tissue+distribution,+and+applications+in+detecting+liver+cancers.">Superparamagnetic iron oxide nanoparticles stabilized by alginate: Pharmacokinetics, tissue distribution, and applications in detecting liver cancers.</a> International Journal of Pharmaceutics. 354 (1-2), 217-226 (2008).</li><li>Wang, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liver+tumors:+Monitoring+embolization+in+rabbits+with+VX2+tumors+-Transcatheter+intraarterial+first-pass+perfusion+MR+imaging.">Liver tumors: Monitoring embolization in rabbits with VX2 tumors -Transcatheter intraarterial first-pass perfusion MR imaging.</a> Radiology. 245 (1), 130-139 (2007).</li><li>Bimonte, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radio-frequency+ablation-based+studies+on+VX2rabbit+models+for+HCC+treatment.">Radio-frequency ablation-based studies on VX2rabbit models for HCC treatment.</a> Infectious Agents and Cancer. 11 (1), (2016).</li><li>Gaba, R., Obeid, M., Khabbaz, R., Garcia, K., Schachtschneider, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translational+Animal+Models+for+Liver+Cancer.">Translational Animal Models for Liver Cancer.</a> American Journal of Interventional Radiology. 2 (2), 1-8 (2018).</li><li>Kuszyk, B. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+tumor+recurrence+following+hepatic+cryoablation:+radiologic-histopathologic+correlation+in+a+rabbit+model.">Local tumor recurrence following hepatic cryoablation: radiologic-histopathologic correlation in a rabbit model.</a> Radiology. 217 (2), 477-486 (2000).</li><li>Geschwind, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemoembolization+of+Liver+Tumor+in+a+Rabbit+Model+Assessment+of+Tumor+Cell+Death+with+Diffusion-Weighted+MR+Imaging+and+Histologic+Analysis.">Chemoembolization of Liver Tumor in a Rabbit Model Assessment of Tumor Cell Death with Diffusion-Weighted MR Imaging and Histologic Analysis.</a> Journal of Vascular and Interventional Radiology. 11 (10), 1245-1255 (2000).</li><li>Virmani, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+Two+Different+Methods+for+Inoculating+VX2+Tumors+in+Rabbit+Livers+and+Hind+Limbs.">Comparison of Two Different Methods for Inoculating VX2 Tumors in Rabbit Livers and Hind Limbs.</a> Journal of Vascular and Interventional Radiology. 19 (6), 931-936 (2008).</li><li>Seldinger, S. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catheter+replacement+of+the+needle+in+percutaneous+arteriography:+A+new+technique.">Catheter replacement of the needle in percutaneous arteriography: A new technique.</a> Acta Radiologica. 49 (SUPPL 434), 47-52 (2008).</li><li>Schook, L. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+genetic+porcine+model+of+cancer.">A genetic porcine model of cancer.</a> PLoS One. 10 (7), e0128864 (2015).</li></ol>

vx2 肿瘤方法的第一个关键步骤是成功地在供体兔的后肢传播肿瘤。有关此步骤的详细信息, 请参阅 "代表性结果" 部分的第一段。
下一个关键步骤是确保正确识别可行的肿瘤胶囊。这不仅对肿瘤悬浮液的制备是必要的, 对肝植入的肿瘤碎片的选择和产生也很重要。图 4对活肿瘤和周围肌肉组织之间的界限进行了注释。如果在悬浮液制备过程中刮伤不正确的...

兔 vx2 肿瘤模型自 1935年 1,2发展以来, 在实验肿瘤学中发挥了重要作用。该肿瘤是一种病毒引起的再生鳞状细胞癌, 其特征是血管增生、生长快、在骨骼肌中容易繁殖 3,4。而兔 vx2 肿瘤模型已被用来研究多种癌症5,6,7,8;本文的重点是肝癌9。
所述方法的目的是提出一个模型的原发性肝癌, 或肝细胞癌 (hcc), 可用于介入放射科医生的翻译研究。它可用于药代动力学研究、治疗调查和消融方法检测10,11,12,13,14,15.
与老鼠、老鼠、土木等老鼠模型或灵长类动物 1 6号等较大模型等其他模型相比, 本文详细介绍的方法具有多重优势.主要好处之一是肿瘤生长迅速可靠, 这使得研究人员能够在第一次后肢繁殖后一个月内建立活跃的肿瘤线.此外, 这种肿瘤有直接的超声表现可见性和高血管的外围, 这允许跨动脉局部治疗和消融治疗。最后, 也是最重要的, 兔子血管的大小允许可行和技术上容易利用血管器械 18.

<table>
	<tbody>
		<tr>
			<td>MethoCult (Methycellulose)</td>
			<td>Stemcell Technologies</td>
			<td>M3134</td>
			<td></td>
		</tr>
		<tr>
			<td>VX2 Cell Line</td>
			<td>NCI</td>
			<td>VX-2</td>
			<td></td>
		</tr>
		<tr>
			<td>5 mL Syringe</td>
			<td>BD</td>
			<td>309646</td>
			<td></td>
		</tr>
		<tr>
			<td>16-Gauge Needle</td>
			<td>BD</td>
			<td>305197</td>
			<td></td>
		</tr>
		<tr>
			<td>22-Gauge Needle</td>
			<td>BD</td>
			<td>305155</td>
			<td></td>
		</tr>
		<tr>
			<td>Hair Clippers</td>
			<td>Wahl</td>
			<td>41870-0438</td>
			<td></td>
		</tr>
		<tr>
			<td>Foam Insulated Box</td>
			<td>Mr. Box Online</td>
			<td>10 x 10 x 4</td>
			<td></td>
		</tr>
		<tr>
			<td>Acepromazine</td>
			<td>Henry Schein</td>
			<td>003845</td>
			<td></td>
		</tr>
		<tr>
			<td>Buprenorphine</td>
			<td>Par</td>
			<td>42023-179-05</td>
			<td></td>
		</tr>
		<tr>
			<td>Meloxicam</td>
			<td>Henry Schein</td>
			<td>049755</td>
			<td></td>
		</tr>
		<tr>
			<td>Alcohol Pads</td>
			<td>Covidien</td>
			<td>5033</td>
			<td></td>
		</tr>
		<tr>
			<td>Ketamine</td>
			<td>Henry Schein</td>
			<td>056344</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylazine</td>
			<td>Akorn</td>
			<td>59399-110-20</td>
			<td></td>
		</tr>
		<tr>
			<td>Pentobarbital (Fatal-Plus)</td>
			<td>Vortech</td>
			<td>9373</td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile Petri Dish</td>
			<td>Thermo Fisher</td>
			<td>172931</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Gibco</td>
			<td>11965092</td>
			<td></td>
		</tr>
		<tr>
			<td>Saline</td>
			<td>Baxter</td>
			<td>2F7124</td>
			<td></td>
		</tr>
		<tr>
			<td>15-Blade</td>
			<td>Steris</td>
			<td>02-050-015</td>
			<td></td>
		</tr>
		<tr>
			<td>Scalpel Handle x 2</td>
			<td>Steris</td>
			<td>22-2381</td>
			<td></td>
		</tr>
		<tr>
			<td>Curved Hemostat</td>
			<td>WPI</td>
			<td>501288</td>
			<td></td>
		</tr>
		<tr>
			<td>Atraumatic Forceps</td>
			<td>Sklar</td>
			<td>52-5077</td>
			<td></td>
		</tr>
		<tr>
			<td>Gauze</td>
			<td>Medline</td>
			<td>NON21430LF</td>
			<td></td>
		</tr>
		<tr>
			<td>11-Blade</td>
			<td>Steris</td>
			<td>02-050-011</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgicel</td>
			<td>Ethicon</td>
			<td>1951</td>
			<td></td>
		</tr>
		<tr>
			<td>3-0 PDS / Taper</td>
			<td>Ethicon</td>
			<td>Z305H</td>
			<td></td>
		</tr>
		<tr>
			<td>4 - 0 Vicryl / Cutting</td>
			<td>Ethicon</td>
			<td>J392H</td>
			<td></td>
		</tr>
		<tr>
			<td>40 micron strainer</td>
			<td>BD</td>
			<td>352340</td>
			<td></td>
		</tr>
		<tr>
			<td>50 mL conical tube</td>
			<td>Thermo Fisher</td>
			<td>339652</td>
			<td></td>
		</tr>
		<tr>
			<td>plastic pipette</td>
			<td>Thomas Scientific</td>
			<td>HS206371B</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge</td>
			<td>Sorvall</td>
			<td>75004240</td>
			<td></td>
		</tr>
		<tr>
			<td>1.40mL Tubes (Internal Thread)</td>
			<td>Micronic</td>
			<td>MP32131-Z20</td>
			<td></td>
		</tr>
		<tr>
			<td>3-F VSI Micro-HV Introducer Kit</td>
			<td>Vascular Solutions</td>
			<td>Custom Order (P15180391)</td>
			<td></td>
		</tr>
		<tr>
			<td>.018 45-degree angle glidewire</td>
			<td>Terumo</td>
			<td>RG*GA1818SA</td>
			<td></td>
		</tr>
		<tr>
			<td>Direxion bern-shape microcatheter</td>
			<td>Boston Scientific</td>
			<td>M001195230</td>
			<td></td>
		</tr>
		<tr>
			<td>Omnipaque</td>
			<td>GE</td>
			<td>Y510</td>
			<td></td>
		</tr>
	</tbody>
</table>

development and angiographic use of the rabbit vx2 model for liver cancer

兔 vx2 肿瘤是介入放射学领域肝细胞癌 (hcc) 转化研究的常用动物模型。该模型采用了一种再生鳞状细胞癌, 该细胞癌在供体家兔的骨骼肌中容易和可靠地传播, 以便最终收获并同种异体移植植入天真受者的肝脏。这种肿瘤移植在受者兔的肝脏中迅速生长成血管造影可识别的肿瘤, 其特征是由一个可行的血管高血管囊包围的坏死核。兔子解剖的物理大小足以促进血管器械, 允许各种介入技术的应用和测试。尽管有这些好处, 但缺乏技术资源, 无法作为研究人员使用该模型的具体参考。在此, 我们提出了一个全面的视觉概述的技术方面的发展, 生长, 繁殖, 和血管造影利用的兔子 vx2 肿瘤模型, 供新手和有经验的研究人员使用。

当看图 1, 很明显, 兔子的四头肌扩大。此外, 多个小的离散结节, 通常与肿瘤生长通过筋膜相关, 是可见的。触诊时, 注射肢体应出现比非注射肢体。如果研究人员需要更明确的肿瘤存在保证, 超声成像可以用来识别嵌入肌肉中的肿瘤。如果没有检测到肿瘤, 后肢应重新注射肿瘤细胞悬浮液。
为了确认成功?...

Watch this Scientific Journal Video about Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer at JoVE.com

Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer

兔 vx2 模型在肝癌中的开发与应用

本文的目的是为 vx2 肝癌兔模型的开发和应用提供一个入门。

The rabbit VX2 tumor is an animal model commonly utilized for translational research regarding hepatocellular carcinoma (HCC) in the field of ...

这种临床前肝肿瘤模型可以帮助回答介入放射学和介入肿瘤学领域局部区域治疗的关键问题。该技术的主要优点是，它通过正交同位素植入产生可靠的肿瘤诱导。对于肿瘤的实现，首先确认对踏板反射缺乏反应。一旦确认，动物主体被放置在麻醉下。确认适当的麻醉水平，腹部无菌准备和披上。麻醉后，使用15号刀片，从西腓过程开始，进行三到四厘米的垂直中线皮肤切口。缩回皮肤，以识别线性阿尔巴，一个反光的白色组织带，沿着中线低劣地传播。并使用钝和尖锐的解剖穿过线阿尔巴， 暴露内膜， 注意不要穿孔下肠道组织.仔细解剖通过腹腔组织，定位肝脏，使用哈莫马特延长中线切口一至两厘米，通过皮肤，肌肉和腹膜，必要时。识别左叶，这是位于中线的中叶的推断。将一块干纱布放在切口的劣质方面，以防止肝脏缩回腹部。在提取的肝脏上放置一块新的湿纱布。选择一到两立方毫米肿瘤组织片，用钳子。并使用11号刀片以45度角将肝脏组织刺破至0.5厘米深的口袋，注意不要穿透肝胶囊的后面。轻轻抬起通风口的刀片，在肝床上创建一个小口袋，将肿瘤放入口袋。将一块止血剂放在肿瘤口袋上，促进血红，并防止肿瘤片弹出。并且，在确认腹腔后，将肝脏返回到腹腔。然后在锥形针上使用三零多二酮缝合，在简单的连续缝合中关闭腹壁。和四个零多糖蛋白910缝合在切割针上连续，亚切线缝合，以关闭皮肤。为了确认肿瘤的放置成功，在腹股沟中使股骨槽出现，并沿着凹槽进行两到三厘米的线性切口。使用钝解剖，定位和分离含有股骨静脉、动脉和神经的股骨束，并使用钝解剖将股骨动脉与包中其他结构分离。将动脉隔离在手术刀手柄上，并使用塞尔丁格技术，以及三个法国介绍器套件将针头引入容器。将导丝插入动脉。并取出针头，小心地将三个法国护套推进到船上。从护套上拆下扩张器和导丝，以完成血管通路。在荧光灯引导下，将导管插入T12级的腹腔躯干，通过普通肝动脉和适当的肝动脉将导管推进到左肝动脉中。当导管到达左肝动脉时，注射对比剂以确认超血管肿瘤的存在，然后可以通过动脉内治疗进行选择。取出导管使用三个零丝缝合线，将股骨动脉紧直，并直插到护套的插入点，小心将结紧到护套上，因为它被撤回以防止出血。然后使用四个零聚乳胶910缝合，在切针下针关闭腹股沟切口。植入后三到四周，肿瘤可以在兔子的左四头肌上宏观地可视化。如证明使用血管造影和注射造影剂，可用于确认肿瘤放置后成功传播。进行尸检后，成功植入的肿瘤应清晰可见。自开发以来，该技术为介入放射学和介入肿瘤学领域的研究人员探索小动物模型的肝肿瘤局部区域疗法铺平了道路。

Research

JoVE Journal

Cancer Research

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

原发肿瘤发展小鼠实验模型和腹膜转移<em&gt;通过</em&gt;原位接种卵巢癌细胞

Epithelial ovarian carcinoma (EOC) is associated with a poor prognosis because it shows peritoneal dissemination. To improve the prognosis, it is ...

科研

癌症研究

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

开发和维护的临床前患者来源的肿瘤异种移植模型为新的抗癌疗法的调查

Patient derived tumor xenograft (PDTX) models provide a necessary platform in facilitating anti-cancer drug development prior to human trials. Human tumor ...

Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones

肝大肠癌转移的高级动物模型：成像技术和转移性克隆性质

Patients with a limited number of hepatic metastases and slow rates of progression can be successfully treated with local treatment approaches1,2. ...

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

门静脉注射模型来研究肝转移乳腺癌

Breast cancer is the leading cause of cancer-related mortality in women worldwide. Liver metastasis is involved in upwards of 30% of cases with breast ...

Evaluation of the Feasibility, Safety, and Accuracy of an Intraoperative High-intensity Focused Ultrasound Device for Treating Liver Metastases

术中高强度聚焦超声装置治疗肝转移的可行性、安全性和准确性评价

Today, the only potentially curative option in patients with colorectal liver metastases is surgery. However, liver resection is feasible in less than 20% ...

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells

矫形子宫内膜癌模型，具有从 Vivo 传播和培养的 VX2 细胞中制成的再生淋巴病

Endometrial cancer is the most common gynecologic malignancy in North America and the incidence is rising worldwide. Treatment consists of surgery with or ...

Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury

缺血再灌注损伤中转移性肝肿瘤的模样

Liver ischemia and reperfusion (I/R) injury, a common clinical challenge, remains an inevitable pathophysiological process that has been shown to induce ...

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

多维共同培养系统模拟肺鳞癌进展

Tumor-stroma interactions play a critical role in the development of lung squamous carcinoma (LUSC). However, understanding how these dynamic interactions ...

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

门静脉注射结直肠癌类器官研究肝转移基质

Hepatic metastasis of colorectal cancer (CRC) is a leading cause of cancer-related death. Cancer-associated fibroblasts (CAFs), a major component of the ...

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

一种三维球体模型研究肝细胞癌肿瘤-基质相互作用

The aggressiveness and lack of well-tolerated and widely effective treatments for advanced hepatocellular carcinoma (HCC), the predominant form of liver ...