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DOI :
10.3791/58627-v
•
10:21 min
February 1st, 2019
Chapters
0:04
Title
0:48
Design of Binding Domains (BDs) of SINEUP Constructs for Target mRNAs
1:48
Cell Culture and SINEUPs Transfection
3:46
Protein Extraction
5:32
Protein Separation by SDS-PAGE and Detection of Target Proteins by Western-blot Analysis
7:21
Imaging Analysis of SINEUPs by Semi-automated Detection Method
8:26
Results: Analysis of Translation Upregulation by SINEUP-GFP
9:11
Conclusion
SINEUPs は、合成アンチセンス非コード Rna 結合ドメイン (BD) とエフェクター ドメイン (ED) 含みの翻訳を調整するターゲット mRNA。ここでは、培養細胞、西部のしみ方と半自動化されたハイスループット イメージング システムによって翻訳推進活動の分析の SINEUPs の検出方法について述べる.
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