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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

DOI :

10.3791/58647-v

January 7th, 2019

January 7th, 2019

7,647 Views

1Department of Pharmacology, Faculty of Dentistry, Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

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