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DOI :
10.3791/58648-v
•
9:43 min
November 30th, 2018
Chapters
0:04
Title
1:06
Preparation of the Sample Extract
2:38
Preparation of the Crude Histone Extract
3:33
Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
4:14
Purification of Core Histones
5:13
Precipitation of Core Histones
7:02
Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
9:02
Conclusion
والغرض من هذه المقالة توفير دليل شامل ومنهجي لفعالية تنقية histones H3 و H4 والتحديد الكمي لبقايا هيستون أسيتيلاتيد.
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