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DOI :
10.3791/58666-v
•
7:22 min
February 11th, 2019
Chapters
0:04
Title
0:27
Preparation of Protein Sample
1:31
Performing a Thermal Shift Assay (TSA) Experiment
3:22
Preparing and Programing a Nano Differential Scanning Fluorimetry (nanoDSF) Experiment
5:35
Results: Analysis of the Temperature at the Inflection Point of the Unfolding Transition (Tm) in Lysozyme
6:55
Conclusion
急速に熱ゲルシフト試金およびナノ示差走査/蛍光定量による条件の様々 なタンパク質の熱安定性をテストするためのプロトコルが表示されます。バッファー システム、塩・添加物、一緒には、構造と機能の研究の適切なバッファーを識別するために蛋白質の試金される 3 つのユニークな安定画面を構成します。
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