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DOI :
10.3791/58697-v
January 3rd, 2019
Chapters
0:04
Title
0:51
Substrate Preparation
1:28
Complex Assembly
2:14
Immobilization of the RNA/protein Complexes on Streptavidin Beads
4:00
UV-Elution
5:08
Sample Analysis by Silver Staining Followed by Mass Spectrometry
5:59
Results: Analysis of UV-eluted Complexes
7:36
Conclusion
采用肉毒分蛋白策略纯化的 rna* 蛋白复合物在变性条件下被洗脱, 以不适合进一步纯化和功能分析的形式进行溶液处理。在这里, 我们描述了这种策略的修改, 利用光裂解链接器在 rna 和温和的紫外线洗脱步骤, 产生本地和全功能的 rna 蛋白复合物。
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