Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/58764-v
•
9:56 min
November 14th, 2018
Chapters
0:04
Title
0:27
Identification and Cloning of Guide RNA Sequence
4:28
Designing and Generation of Repair Template
5:33
Transformation of C. albicans with Repair Template and Plasmid
7:47
Colony PCR
8:52
Results: Evaluation of Successful Genome Editing in C. albicans
9:27
Conclusion
カンジダ ・ アルビカンスの効率的なゲノム工学は病態解明と治療法の開発を理解する重要です。ここでは、迅速かつ正確に CRISPR を使用してc. アルビカンスゲノムを編集するプロトコルについて述べる。プロトコルでは、研究者の点突然変異、挿入、および削除を含む遺伝の修正のいろいろを紹介することができます。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved