Name: one-anastomosis gastric bypass (oagb) in rats
Uploaded: 2018-11-10T13:00:00
Description: Watch this Scientific Journal Video about One-anastomosis Gastric Bypass OAGB in Rats at JoVE.com

作者要感谢让·皮埃尔·马穆塞的不断帮助和支持;eglantine voitellier、jean-baptiste cavin 和 houneyda el jindi 用于设置模型和插图;安妮-夏洛特·贾里和安德烈亚贡卡尔维斯帮助他们的视频;sara j. zaat 编辑英文文本。matthieu a. siebert 和 lara ribero-parorente 是 mécherchémale 基金会 (frm) 和法国奇鲁吉协会 (afc) 的赞助者。

所有动物用途都符合欧洲共同体的准则, 并得到了地方伦理委员会 (第121号国家的动物扩展委员会) 和高等教育和研究部 (参考 #02285. 03) 的批准。
1. 动物和饮食
<ol><li>
大 鼠
	<ol>
<li>使用体重260-320 克的雄性 wistar 大鼠, 在7周大的时候开始高脂肪饮食 (hfd)。</li>
	</ol>
</li>
	<li>
饮食
	<ol>
<li>导致饮食引起的肥胖, 至少有12个术前的 hfd 周。让老鼠免费获得水...

<ol>
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Consensus conference statement bariatric surgery for morbid obesity: health implications for patients, health professionals, and third-party payers.</a> Surgery for Obesity and Related Disease. 1, 371-381 (2005).</li><li>Mingrone, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bariatric-metabolic+surgery+versus+conventional+medical+treatment+in+obese+patients+with+type+2+diabetes:+5+year+follow-up+of+an+open-label,+single-centre,+randomised+controlled+trial.">Bariatric-metabolic surgery versus conventional medical treatment in obese patients with type 2 diabetes: 5 year follow-up of an open-label, single-centre, randomised controlled trial.</a> Lancet. 386, 964-973 (2015).</li><li>Nguyen, N. T., Varela, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bariatric+surgery+for+obesity+and+metabolic+disorders:+state+of+the+art.">Bariatric surgery for obesity and metabolic disorders: state of the art.</a> Nature Reviews. Gastroenterology &amp; Hepatology. 14, 160-169 (2017).</li><li>Rutledge, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mini-gastric+bypass:+experience+with+the+first+1,274+cases.">The mini-gastric bypass: experience with the first 1,274 cases.</a> Obesity Surgery. 11, 276-280 (2001).</li><li>Lee, W. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+Roux-en-Y+vs.+mini-gastric+bypass+for+the+treatment+of+morbid+obesity:+a+10-year+experience.">Laparoscopic Roux-en-Y vs. mini-gastric bypass for the treatment of morbid obesity: a 10-year experience.</a> Obesity Surgery. 22, 1827-1834 (2012).</li><li>Victorzon, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-anastomosis+gastric+bypass:+better,+faster,+and+safer?.">Single-anastomosis gastric bypass: better, faster, and safer?.</a> Scandinavia Journal of Surgery. 104, 48-53 (2015).</li><li>Magouliotis, D. E., Tasiopoulou, V. S., Tzovaras, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=One+anastomosis+gastric+bypass+versus+Roux-en-Y+gastric+bypass+for+morbid+obesity:+a+meta-analysis.">One anastomosis gastric bypass versus Roux-en-Y gastric bypass for morbid obesity: a meta-analysis.</a> Clinical Obesity. 8, 159-169 (2018).</li><li>Seyfried, F., le Roux, C. W., Bueter, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lessons+learned+from+gastric+bypass+operations+in+rats.">Lessons learned from gastric bypass operations in rats.</a> Obesity Facts. 4 Suppl 1, 3-12 (2011).</li><li>Wang, F. G., Yu, Z. P., Yan, W. M., Yan, M., Song, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+safety+and+effectiveness+between+laparoscopic+mini-gastric+bypass+and+laparoscopic+sleeve+gastrectomy:+A+meta-analysis+and+systematic+review.">Comparison of safety and effectiveness between laparoscopic mini-gastric bypass and laparoscopic sleeve gastrectomy: A meta-analysis and systematic review.</a> Medicine (Baltimore). 96, e8924 (2017).</li><li>Hong, J., Park, S., Menzo, E. L., Rosenthal, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Midterm+outcomes+of+laparoscopic+sleeve+gastrectomy+as+a+stand-alone+procedure+in+super-obese+patients.">Midterm outcomes of laparoscopic sleeve gastrectomy as a stand-alone procedure in super-obese patients.</a> Surgery for Obesity and Related Disease. 14 (3), 297-303 (2017).</li><li>Abellan, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+influence+of+the+percentage+of+the+common+limb+in+weight+loss+and+nutritional+alterations+after+laparoscopic+gastric+bypass.">The influence of the percentage of the common limb in weight loss and nutritional alterations after laparoscopic gastric bypass.</a> Surgery for Obesity Related Disease. 10, 829-833 (2014).</li><li>Nora, M., Morais, T., Almeida, R., Guimar&#227;es, M., Monteiro, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Should+Roux-en-Y+gastric+bypass+biliopancreatic+limb+length+be+tailored+to+achieve+improved+diabetes+outcomes?.">Should Roux-en-Y gastric bypass biliopancreatic limb length be tailored to achieve improved diabetes outcomes?.</a> Medicine (Baltimore). 96, e8859 (2017).</li><li>Madan, A. K., Harper, J. L., Tichansky, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+of+laparoscopic+gastric+bypass:+on-line+survey+of+American+Society+for+Bariatric+Surgery+practicing+surgeons.">Techniques of laparoscopic gastric bypass: on-line survey of American Society for Bariatric Surgery practicing surgeons.</a> Surgery for Obesity and Related Disease. 4, 166-173 (2008).</li><li>Himpens, J. M., Vilallonga, R., Cadi&#232;re, G. B., Leman, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+consequences+of+the+incorporation+of+a+Roux+limb+in+an+omega+loop+(mini)+gastric+bypass:+evaluation+by+a+glucose+tolerance+test+at+mid-term+follow-up.">Metabolic consequences of the incorporation of a Roux limb in an omega loop (mini) gastric bypass: evaluation by a glucose tolerance test at mid-term follow-up.</a> Surgical Endoscopy. 30, 2935-2945 (2016).</li><li>Parmar, C. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mini+Gastric+Bypass:+first+report+of+125+consecutive+cases+from+United+Kingdom.">Mini Gastric Bypass: first report of 125 consecutive cases from United Kingdom.</a> Clinical Obesity. 6, 61-67 (2016).</li><li>Chevallier, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=One+thousand+single+anastomosis+(omega+loop)+gastric+bypasses+to+treat+morbid+obesity+in+a+7-year+period:+outcomes+show+few+complications+and+good+efficacy.">One thousand single anastomosis (omega loop) gastric bypasses to treat morbid obesity in a 7-year period: outcomes show few complications and good efficacy.</a> Obesity Surgery. 25, 951-958 (2015).</li><li>Kular, K. S., Manchanda, N., Rutledge, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+6-year+experience+with+1,054+mini-gastric+bypasses-first+study+from+Indian+subcontinent.">A 6-year experience with 1,054 mini-gastric bypasses-first study from Indian subcontinent.</a> Obesity Surgery. 24, 1430-1435 (2014).</li><li>Tacchino, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bowel+length:+measurement,+predictors,+and+impact+on+bariatric+and+metabolic+surgery.">Bowel length: measurement, predictors, and impact on bariatric and metabolic surgery.</a> Surgery for Obesity Related Disease. 11, 328-334 (2015).</li><li>Bruinsma, B. G., Uygun, K., Yarmush, M. L., Saeidi, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+models+of+Roux-en-Y+gastric+bypass+surgery+and+sleeve+gastrectomy+in+rats+and+mice.">Surgical models of Roux-en-Y gastric bypass surgery and sleeve gastrectomy in rats and mice.</a> Nature Protocols. 10, 495-507 (2015).</li><li>Jeppsson, B., Mangell, P., Thorlacius, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+probiotics+as+prophylaxis+for+postoperative+infections.">Use of probiotics as prophylaxis for postoperative infections.</a> Nutrients. 3, 604-612 (2011).</li><li>Cavin, J. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differences+in+Alimentary+Glucose+Absorption+and+Intestinal+Disposal+of+Blood+Glucose+After+Roux-en-Y+Gastric+Bypass+vs+Sleeve+Gastrectomy.">Differences in Alimentary Glucose Absorption and Intestinal Disposal of Blood Glucose After Roux-en-Y Gastric Bypass vs Sleeve Gastrectomy.</a> Gastroenterology. 150, 454-464 (2016).</li><li>Bruzzi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-Term+Evaluation+of+Biliary+Reflux+After+Experimental+One-Anastomosis+Gastric+Bypass+in+Rats.">Long-Term Evaluation of Biliary Reflux After Experimental One-Anastomosis Gastric Bypass in Rats.</a> Obesity Surgery. 27, 1119-1122 (2017).</li><li>Cavin, J. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Malabsorption+and+intestinal+adaptation+after+one+anastomosis+gastric+bypass+compared+with+Roux-en-Y+gastric+bypass+in+rats.">Malabsorption and intestinal adaptation after one anastomosis gastric bypass compared with Roux-en-Y gastric bypass in rats.</a> American Journal of Physiology-Gastrointestinal and Liver Physiology. 311, G492-G500 (2016).</li><li>Jacob, P. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isocaloric+intake+of+a+high-fat+diet+promotes+insulin+resistance+and+inflammation+in+Wistar+rats.">Isocaloric intake of a high-fat diet promotes insulin resistance and inflammation in Wistar rats.</a> Cell Biochemistry and Function. 31, 244-253 (2013).</li></ol>

该协议的优点是模仿 oagb 在人类中的常规做法。它在大鼠体内的安全性和重现性已在临床和代谢结果中得到证明。
该协议中的吻合带来了技术难题, 是主要的关键一步。为此, 有必要使用特定的显微外科工具, 如显微外科针架或放大镜。为了实现没有任何狭窄的密封吻合, 有必要在吻合的每一侧进行至少七到八个紧密缝合, 并避免在任何缝合中采取任何粘膜, 以便将其埋在缝合?...

病态肥胖是西方世界的一个主要健康问题, 发病率不断上升 1.减肥手术仍然是治疗病态肥胖2的唯一长期有效治疗方法, 对肥胖相关的合群3有明显的好处.roux-en-y 胃旁路 (rygb) 仍然是黄金标准, 因为它是最古老的程序之一, 也是世界上执行次数最多的手术之一, 随访时间最长4。oagb 是一个较新的程序, 最早描述在2001年由 rutledge5。它被描述为 rygb6 的有效替代品, 并有一些优势。早期数据显示, 在减肥和解决与肥胖有关的合感 6、7方面, 与 rygb 具有类似的功效。此外, 避免了 rygb 空肠空肠吻合术的狭窄或渗漏风险, 内部疝的风险可能低于 rygb8。
减肥手术后体重下降的生理机制仍有争议, 在人类中可能难以研究9。动物模型是有用的, 以了解如何减肥手术可能会影响葡萄糖代谢, 神经内分泌修改, 或饱足机制。程序之间缺乏比较数据, 需要更好地了解这些手术的长期效果.不可能完全规范术前和术后的临床护理, 令人满意的随访可能很难实现: 一些研究报告说, 第一年11的损失到随访率高达30%。
胃旁路的概念是一个限制性程序的组合, 由于创建胃袋, 和适度的吸收不良, 由于排除十二指肠和近端空肠胃肠道转运。目前, 减肥手术中的肢体长度仍是争论的 1 2日、1 3.现有数据显示, 程序的差异很大, 尚未就 oagb14,15的最佳肢体长度达成共识。尽管如此, 许多作者报告说, oagb 16、17、18的标准为 200厘米 bpl 长度。小肠的平均长度约为700厘米19。
在全球20个国家开发了几个啮齿类动物模型, 在眼袋大小、肢体长度和迷走神经保存方面存在很大差异。但很少有作者报告实验 oagb 模型;为此, 研究小组与减肥外科医生合作, 开发了 oagb 模型。该模型开发背后的原理是模仿在人类中练习的胃旁路。该模型结合了胃袋的结构与排除的内部和胃的身体。为此, 外科医生使用订书装置对非腺性胃进行消融。与之前的第20号手术相比, 研究小组决定增加预防性抗生素治疗, 以降低与胃肠道吻合口相关的发病率, 就像人类手术中的常规一样21。
该模型中使用的大鼠肢体长度是为了再现人类 bpl 和 cl 的比例。初步测量显示, 大鼠小肠总长度在90至120厘米22之间.因此, 为 oagb 选择的 bpl 长度为35厘米。此外, 该模型是可行的, 无需任何血管结扎, 避免胃袋缺血。

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	<tbody>
		<tr height="62">
			<td height="62" style="height:62px;width:223px;">High-Fat Diet, 45 % fat&#160;</td>
			<td style="width:73px;">Genestil, Royaucourt, France</td>
			<td style="width:123px;">C 1090-45</td>
			<td style="width:172px;">http://www.genestil.com/</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;">Metzenbaum Scissors</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td align="right">501739</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;">Dumont Tweezers</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td>14098</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;width:223px;">Iris Forceps</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td>15915</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;width:223px;">Iris Scissors 10cm</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td>14218</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;">Needle Holder</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td>14110</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;">Alm Self Retaining Retractor</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td>14240</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;">Baby Mixter Hemostatic Forceps,&#160; Right Angle</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td style="width:123px;">501240</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="104">
			<td height="104" style="height:104px;">Non woven sterile swabs</td>
			<td style="width:73px;">LCH Medical Products, Paris, France</td>
			<td>SN40-0755</td>
			<td>www.lch-medical.com</td>
		</tr>
		<tr height="125">
			<td height="125" style="height:125px;width:223px;">Galilean Binocular Loupe 2.5x</td>
			<td style="width:73px;">World Precision instrument, Sarasota, US</td>
			<td style="width:123px;">504056</td>
			<td>https://www.wpi-europe.com</td>
		</tr>
		<tr height="104">
			<td height="104" style="height:104px;">Digestive thread: Isotactic polypropylene, monofil Prolene 7-0&#160;</td>
			<td style="width:73px;">Ethicon, Issy les&#160; Moulineaux, France</td>
			<td style="width:123px;">EH7813E</td>
			<td>www.ethicon.com</td>
		</tr>
		<tr height="83">
			<td height="83" style="height:83px;">Parietal thread : Coated Braided Polyester Ti-Cron 2-0</td>
			<td style="width:73px;">Covidien, Mannsfield, MA, USA</td>
			<td>3003-52</td>
			<td>www.covidien.com</td>
		</tr>
		<tr height="104">
			<td height="104" style="height:104px;">Cutaneous thread : Polyglactin, VICRYL RAPIDE 4-0&#160;</td>
			<td style="width:73px;">Ethicon, Issy les&#160; Moulineaux, France</td>
			<td>VR3100</td>
			<td>www.ethicon.com</td>
		</tr>
		<tr height="104">
			<td height="104" style="height:104px;">ETS-Flex 35- mm staple gun</td>
			<td style="width:73px;">Ethicon, Issy les&#160; Moulineaux, France</td>
			<td style="width:123px;">ATS45</td>
			<td>www.ethicon.com</td>
		</tr>
		<tr height="104">
			<td height="104" style="height:104px;">Proximate Reloadable Staplers</td>
			<td style="width:73px;">Ethicon, Issy les&#160; Moulineaux, France</td>
			<td>XR30V</td>
			<td>www.ethicon.com</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">TA-DST 30mm-3.5mm</td>
			<td>Ethicon, Issy les&#160; Moulineaux, France</td>
			<td>TX30B</td>
			<td>www.ethicon.com</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Alcoholic 5% Betadine</td>
			<td>MEDA Pharma, Merignac, France</td>
			<td>41085</td>
			<td>www.medapharma.fr</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">LIDOCAINE AGUETTANT 20 mg/ml</td>
			<td>LABORATOIRE AGUETTANT, Lyon, France</td>
			<td>3400940000000.0</td>
			<td>https://www.aguettant.fr</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Penicillin G 5MUI</td>
			<td>Panpharma, Luitre, France</td>
			<td>3.40E+12</td>
			<td>https://www.panpharma.eu/fr</td>
		</tr>
		<tr height="62">
			<td height="62" style="height:62px;">Bionolyte G5 ; Sodium chloride 0.4%, glucose 5%, potassium chloride 0.2%)</td>
			<td style="width:73px;">Baxter, Maurepas, France</td>
			<td style="width:123px;"></td>
			<td></td>
		</tr>
		<tr height="62">
			<td height="62" style="height:62px;width:223px;">Liquid diet</td>
			<td style="width:73px;">Genestil, Royaucourt, France</td>
			<td style="width:123px;">C-0200T</td>
			<td>http://www.genestil.com/</td>
		</tr>
		<tr height="83">
			<td height="83" style="height:83px;width:223px;">Isoflurane 100%</td>
			<td style="width:73px;">Centravet, Plancoet, France</td>
			<td style="width:123px;">ISO007</td>
			<td>http://cooperative.centravet.net</td>
		</tr>
	</tbody>
</table>

one-anastomosis gastric bypass (oagb) in rats

该方案的目标是建立一个肥胖大鼠的临床前减肥手术模型, 更具体地说, 是 oagb。基于这一临床前模型, 可以进行纵向研究, 以提供更好地了解的机制背后的结果后看到的减肥手术后, 在人类。为此, 大鼠在全身麻醉下使用异氟醚进行剖腹手术。首先, 外科医生创建一个长的和管状的胃袋: 在更大的曲线和裂孔解剖后, 非腺胃被钉和删除。然后, 剩余的胃也被钉在一起, 以创建胃管, 并排除胃的肛门。之后, 外科医生从十二指肠空肠角进行一个端侧胃空肠造口术35厘米。选择这种肢体长度是为了复制与人类减肥手术相同的双肢肢体 (bpl) 长度和普通肢体长度之间的比率。手术结束时, 通过神经和皮肤闭合。术后早期的管理包括皮下注射水合作用、肌肉内预防性抗生素注射、异洛卡因顶叶注射、止痛药的服用和饮食的逐步重新引入。

该协议是可重现的, 并模仿 oagb 在人类身上的实践。发布的数据23、24 支持该协议的安全性, 死亡率在12% 至25% 之间。研究小组中死亡率相似的不同外科医生都采用了这一方案。
尽管如此, 学习啮齿类动物手术是复杂的, 使用放大镜是强制性的。事实上, 微观吻合术的关键步骤需要大约1...

Watch this Scientific Journal Video about One-anastomosis Gastric Bypass OAGB in Rats at JoVE.com

One-anastomosis Gastric Bypass OAGB in Rats

大鼠胃旁路 (oagb) 的一次吻合

该方案适用于大鼠胃旁路 (oagb) 的表现。操作人员执行一个长的和管状钉胃袋, 然后是手工缝合吻合。对于这种模型, 操作人员在人类中再现了双胰腺和普通肢体之间的相同比例;因此, 胆肠胰腺肢体的尺寸为35厘米。

The goal of this protocol is to set up a preclinical model of bariatric surgery and, more specifically, OAGB in obese rats. Based on this preclinical ...

病态肥胖是西方世界一个主要的健康问题，患病率越来越高。减肥手术仍然是它的唯一长期有效的治疗，在所有的手术，一个阿胃硬化旁路，这是最近的程序似乎是有效和安全的。但是，有几个问题仍未解决。特别是关于手术后对胃粘膜的代谢和长期后果。据我们所知，目前还没有开发一种胃通路旁路的动物模型。这可能有助于研究，例如，葡萄糖代谢，神经元的修饰是长期后果的其他胃粘膜。本视频中介绍的模型是为了模仿人类程序而开发的。首先，外科医生实现长和管状胃的方法，然后绕过35厘米的小肠。此长度已确定，以重现双板肢体和普通肢体之间的比例。就像人类手术一样在此协议中使用 Wistar 大鼠。并使用饮食和只是肥胖与至少12亲身体周的高脂肪饮食。确保大鼠在手术时肥胖。重量在600到800克之间。这里是介绍手术器械，这是这个程序所需的。手术前将所有手术器械都去处理。在空气中吸入3%异氟兰的老鼠麻醉，以在红盒中指示。然后将大鼠转移到加热板，并保持麻醉与面罩在相同的流速3%手术方法是一个梅吉翁拉帕雷塔。沿线性阿尔巴切割腹膜，以打开腹腔。大胃曲线从腺体和非腺胃的交界处解剖到腹部的左侧。胃肠逐渐凝固，以解放大胃曲线到他的角度。然后，内侧区域被10个韧带的一部分露出来。腹腔被解剖，同时保存左胃动脉和血管神经。手术订书机水平应用于胃食管结左侧腺体和非腺胃之间的交界处。切除非腺胃后，此胃被移除。然后将第二个带 TA 设备的订书机应用于第一个订书机。其结果是一个小的和管状的胃方法与排除的胃和身体。Duodenojejunal 角位于外科医生测量 35 厘米到未来的蚂蚁。在十二指肠的消性方面进行。然后，操作者进行双体胃切除术，然后用2个运行的偶极素7000的线状缝合进行进一侧胃肠切除术。成功的关键是实现这种在放大镜下进行解剖学，并在单原子症的每一侧执行约7-8个封闭的额外缝合线。其结果是，通过这种单体肌病，一个双胰腺和一个共性肢体连接到胃袋。在手术结束时，外科医生将腹部器官更换到生理位置，然后用泰伦2-0的运行缝合关闭腹壁。然后形成一个渗透 1 possn zilochine ado 这 1 英里公斤， 然后关闭皮肤与运行缝合的 vicryl 快速 4 - 0 执行和内特鲁姆学校注射 20， 0 单位公斤的廉价异烯和上一个光库执行同位素的子注射。1个月后观察到，在这种情况下，大鼠体重减轻了约20%。在以往的经验中，与假手术相比，它明显和持续地减轻了体重。牺牲后，没有修复袋和排除的胃尚未观察到。该程序产生类似的代谢轮廓后，胃旁路在人类。经过一轮测试后，观察，以及更快地返回到基础血症相比，与假老鼠。此外，每次测试后，胰岛素敏感性增加。这个模型的主要优点是密切模仿人类的程序。它是可重复的，并且已经由几个外科医生执行，低 meticula 重量约为 15% 此协议的主要困难是胃膜瘤在我们的经验中，学习曲线需要大约 10 个手术才能有效地执行。谢谢你的关注。

Research

JoVE Journal

Medicine

One-anastomosis Gastric Bypass (OAGB) in Rats

Acute Myocardial Infarction in Rats

大鼠急性心肌梗死

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

科研

医学

Roux-en-Y Gastric Bypass Operation in Rats

空肠Roux-en-Y胃搭桥手术大鼠

Currently, the most effective therapy for the treatment of morbid obesity to induce significant and maintained body weight loss with a proven mortality ...

Orthotopic Liver Transplantation in Rats

大鼠原位肝移植

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Intraoperative Gastroscopy for Tumor Localization in Laparoscopic Surgery for Gastric Adenocarcinoma

胃镜检查术在腹腔镜手术的肿瘤定位于胃腺癌

Determining resection margins for gastric cancer, which are not exposed to the serosal surface of the stomach, is the most important procedure during ...

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency

静脉介入型号：研究旁路移植通畅一个合适的模型

Bypass grafting is an established treatment method for coronary artery disease. Graft patency continues to be the Achilles heel of saphenous vein grafts. ...

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

袖胃切除术的技术和改良的小鼠空肠Roux-en-Y胃旁路

Obesity is a major public health issue, with a prevalence of 4 to 28% for men and 6.2 to 36.5% for women in Europe (from 2003 to 2008). Morbid obesity is ...

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

小鼠体外循环模型: 一种新方法

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...

Preparation of Decellularized Kidney Scaffolds in Rats

在大鼠中准备去细胞化肾脏脚手架

Tissue engineering is a cutting-edge discipline in biomedicine. Cell culture techniques can be applied for regeneration of functional tissues and organs ...

A Protocol for Roux-en-Y Gastric Bypass in Rats using Linear Staplers

使用线性压花器在老鼠身上进行胃旁路的规程

Roux-en-Y gastric bypass (RYGB) is commonly performed for the treatment of severe obesity and type 2 diabetes. However, the mechanism of weight loss and ...

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

小鼠单吻合十二指肠回肠旁路与袖状胃切除术模型

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes ...