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DOI :
10.3791/58790-v
January 17th, 2019
Chapters
0:04
Title
0:37
Selection of Neomycin Resistant Clones
3:41
Screening of Neomycin Resistant Clones
5:34
Elimination of the Neomycin Resistance Gene from the MS2 Cassette Integrated at Subtelomere 15q
6:06
Visualization of TERRA-MS2 Transcripts in Living Cells
6:49
Results: Cancer Cell Clones with MS2 Sequence Tag Created and Imaged
8:17
Conclusion
ここでは、単一 subtelomere で MS2 シーケンス タグを含む癌細胞のクローンを生成するためのプロトコルを提案する.MS2 GFP システムに依存して、このアプローチは、内因性の転写産物のテロメア繰り返し含む RNA (テラ) 生きている細胞の単一テロメアから表現の可視化を実現。 にします。
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