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DOI :
10.3791/58814-v
•
9:59 min
December 5th, 2018
Chapters
0:04
Title
0:51
Bioreactor Flask Cell Culture
1:52
Conditioned Medium (CM) Harvesting from the Bioreactor Flask
2:41
Exosome Isolation onto a Sucrose Cushion
5:02
siRNA Encapsulation into Exosomes by Electroporation
6:11
Removal of Free siRNA Using Size Exclusion Chromatography (SEC)
7:17
Results: Effective Intracellular Delivery of siRNA Using siRNA-encapsulated Exosomes
9:11
Conclusion
外显子是新一代的药物输送载体。我们建立了一个具有高产量和纯度的非连续分离协议, 用于 sirna 的传递。我们还将荧光标记的非特异性 sirna 封装到外显子体中, 并研究了癌细胞中含有 sirna 的外显子体的细胞吸收情况。
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