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DOI :
10.3791/58882-v
February 25th, 2019
Chapters
0:04
Title
0:50
Cell Growth
3:30
Crude Cell Extract Preparation
5:30
Cell-Free Protein Synthesis Batch Format Reactions
6:25
Quantification of the Reporter Protein, [sfGFP]
7:24
Results: Modification and Optimization of the Cell-Free Platform Conditions
9:01
Conclusion
该协议详细介绍了在4天或更短的时间内生成大肠杆菌基细胞提取物并实施体外蛋白质合成反应所需的步骤、成本和设备。为了将这一平台的灵活性用于广泛的应用, 我们讨论了可以调整和优化的反应条件。
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