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DOI :
10.3791/58896-v
February 21st, 2019
Chapters
0:04
Title
0:28
Preparation of a Lipid/UQ-10/FDLL Mix
1:26
Reconstitution of Cytochrome bo3
5:06
Fabrication of Semi-Transparent Gold Electrodes and Modification of the Gold Surface with Self-Assembled Monolayer (SAM)
6:07
Electrochemical Testing of Proteoliposomes Activity
7:46
Detection of Enzymatic Proton Pumping by Fluorescence Microscopy
9:44
Results: Liposome Identification and pH Change
11:35
Conclusion
ここでは、プロトコルを提案単一リポソームの脂質膜を渡るプロトン透過の分子機構の研究例としてシトクロムbo3を使用します。電気化学、蛍光顕微鏡、単一小胞、含む単一または複数の酵素のルーメン内の pH の変化を組み合わせることを検出および分析できる個別に。
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