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DOI :
10.3791/58953-v
January 17th, 2019
Chapters
0:04
Title
0:33
Prepare Cryostat for Tissue Sectioning
1:51
Sectioning Tissue
3:50
Fixing Tissue/Stain-less Visualization
4:47
Laser Capture Microdissection of Purkinje Cells
6:09
Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
7:59
Conclusion
该协议采用无污渍的方法, 通过激光捕获微解剖, 将新鲜冷冻组织中的 purkinje 细胞从人类死后的小脑中观察和分离。该协议的目的是为 rna 测序生成足够数量的高质量 rna。
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