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DOI :
10.3791/58953-v
January 17th, 2019
Chapters
0:04
Title
0:33
Prepare Cryostat for Tissue Sectioning
1:51
Sectioning Tissue
3:50
Fixing Tissue/Stain-less Visualization
4:47
Laser Capture Microdissection of Purkinje Cells
6:09
Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
7:59
Conclusion
このプロトコルは、視覚化し、キャプチャ レーザーマイクロダイ死後人間小脳から新鮮凍結組織におけるプルキンエ細胞を分離する染色無料のアプローチを使用します。このプロトコルの目的は、RNA 配列のため質の高い RNA の十分な量を生成することです。
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