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DOI :
10.3791/58954-v
•
10:34 min
March 15th, 2019
Chapters
0:04
Title
1:24
High-molecular Weight (HMW) DNA Extraction
3:11
Ultra-long DNA Library Construction - Mechanical Shearing-based Protocol
5:19
Ultra-long DNA Library Construction - Transposase Fragmentation-based Protocol
6:23
Sequencing on the Nanopore Device
7:52
Results: Ultra-long DNA Sequencing Protocol
9:38
Conclusion
长读序列极大地促进了复杂基因组的组合和结构变异的表征。我们描述了一种利用纳米粒子测序平台生成超长序列的方法。该方法采用优化的 DNA 提取, 然后进行改进的文库准备, 从人体细胞中产生数百条覆盖适度的千列酶读数。
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