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DOI :
10.3791/58975-v
June 11th, 2019
Chapters
0:04
Title
0:45
Preparation of Differentiation Media
2:04
Seed hPSCs onto Plates at Defined Densities for Differentiation
4:39
Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
5:37
Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
6:58
Conclusion
このプロトコルは、ヒト多能性幹細胞(hPSC)から肝細胞様細胞を18日間で効率的に生成する単層、血清フリー法を詳細に述べている。これは、hPSCが肝細胞様細胞を形成する前に、原始的な筋、決定的な内因性内因性、後部前腸および肝芽前駆体のような中間細胞型に順次分化するように6つのステップを伴う。
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