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Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

DOI :

10.3791/59010-v

8:40 min

March 1st, 2019

March 1st, 2019

57,984 Views

1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School

Here, we present a protocol to generate pseudotyped particles in a BSL-2 setting incorporating the spike protein of the highly pathogenic viruses Middle East respiratory syndrome and severe acute respiratory syndrome coronaviruses. These pseudotyped particles contain a luciferase reporter gene allowing quantification of virus entry into target host cells.

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Pseudotyped Particles

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