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DOI :
10.3791/59010-v
•
8:40 min
March 1st, 2019
Chapters
0:04
Title
0:41
Cell Seeding for Pseudotyped Particle Production
2:05
Three-plasmid Co-transfection
3:53
Pseudotyped Particles Collection
4:46
Pseudotyped Particle Infection
5:54
Infectivity Quantification by Luciferase Assay Readout
7:04
Results: Infectivity Assays of SARS-CoV S and MERS-CoV S Pseudotyped Particles
8:02
Conclusion
ここでは、高病原性ウイルス中東呼吸器症候群、重症急性呼吸器症候群コロナのスパイク ・ プロテインを組み込む BSL 2 設定で新規のパーティクルを生成するプロトコルを提案する.これら新規粒子には、ターゲット ホストの細胞にウイルスのエントリの定量化を許可するルシフェラーゼ レポーター遺伝子が含まれています。
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