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DOI :
10.3791/59021-v
January 30th, 2019
Chapters
0:04
Title
0:31
32P-labeled Primer Labeling for In Vitro Selective 2‘-hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
3:44
Small Molecule Binding and RNA 2‘-OH Modification
6:29
Marker Preparation and Primer Extension
8:59
PAGE Analysis
10:15
Results of In Vitro and In-cell SHAPE for Analyzing Small Molecule Induced Pre-mRNA Structural Changes
11:02
Conclusion
둘 다 생체 외에서 그리고 세포에서 선택적 2'-히 드 록 acylation 뇌관 연장 (모양) 실험 사전 mRNA 시퀀스는 RNA를 대상으로 작은 분자의 존재의 2 차 구조를 결정 하 여 분석에 대 한 자세한 프로토콜은 이 문서에서 제시 하는.
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