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DOI :
10.3791/59033-v
•
8:46 min
November 12th, 2019
Chapters
0:04
Title
0:30
Chimeric Antigen Receptor (CAR)-Encoding Lentivirus Generation
2:29
CAR T Cell Generation and Expansion
3:16
CAR Expression Detection
4:55
Real-Time Cytolysis Potency Assay
6:48
Results: Representative CAR T Cell Target Cell Killing Efficacy as Assessed by RTCA
8:05
Conclusion
液体および固形腫瘍細胞を標的とするキメラ抗原受容体T細胞の効力を評価する定量的なインビトロ細胞質アッセイシステムを説明する。このプロトコルは、他の免疫エフェクター細胞、ならびに組み合わせ治療を評価するために拡張することができる。
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