Name: application of biochip microfluidic technology to detect serum allergen-specific immunoglobulin e (sige)
Uploaded: 2019-04-21T14:00:00
Description: Watch this Scientific Journal Video about Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE at JoVE.com

作者感谢梅江教授在统计分析方面的帮助, 并感谢徐哈默先生在手稿的编写方面的帮助。本研究得到了广州科学技术基金会 (201804020043) 和国家自然科学基金 (国家自然科学基金81572063和国家自然科学基金 81572063) 的支持。供资小组同意研究设计、数据分析、手稿准备和出版决定。没有收到这项研究的其他资金。

本研究和人体血清样本的使用得到了广州医科大学第一附属医院伦理委员会 (GYYY-2016-73) 的批准。所有参与者都独立或通过其父母 (就儿童而言) 给予书面同意。
1. 研究小组的基本资料
注: 呼吸道疾病国家重点实验室 (AIR-SKLRD) 过敏信息库是在广州呼吸医院研究所 (GIRH) 内建立的大型血清库。Air-sklrd 在过去十年中启动, 已开始收集和储存过敏性疾病患者的血清?...

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与许多其他研究 15、16、17的结果类似, 基于293名过敏患者血清的微流控系统的结果显示, 室内尘螨 (包括d. 翼状肌瘤), D. farinae和b. tropicalis) 是导致中国南方过敏性疾病的主要吸入性过敏原, 而对于食物、牛奶、花生、虾和螃蟹来说, 则是导致过敏性症状的最常见的过敏原。关于对三种过敏原进行的重现性研究...

在过去几十年里, 过敏的流行率稳步上升, 影响到全球人口的20% 至 30% 1。病因过敏原的鉴定对疾病的管理具有重要意义。在中国, 由于国内没有登记的体内皮肤刺试验, 体外测定血清特异性 IgE 是 i 型过敏诊断最重要、最常用的工具 2.这与西方世界的做法类似, 但尽管作为荧光酶联免疫吸附基系统的免疫 cap 系统 (系统 1) 被认为是体外过敏诊断的黄金标准 3, 但由于在中国, 其使用非常有限。其较高的设备和试剂价格。因此, 迫切需要一个具有较高性价比的新型替代过敏诊断系统。
BioIC 系统 (系统 2) 是一种基于化学发光原理的基于血清特定 ige 多路检测的微流体墨盒系统。微流体盒的尺寸为7厘米 x 4 厘米, 由三层注塑塑料组成。上部为3毫米厚的透明聚碳酸酯, 在热装配过程中具有良好的稳定性。它与由丙烯腈、丁烯和苯乙烯 (ABS) 的共聚物制成的3毫米厚的底层一起, 将由硅橡胶制成的0.5 毫米厚的中间层三明治。中间层是黑色的, 在化学发光检测过程中提供较低的背景。在硅胶的顶部, 在与反应区相对应的位置喷洒一层薄薄的硝化纤维素膜 (NC 膜), 从而可以发现不同的过敏性蛋白质。本研究的目的是评价微流体系统在血清中多联检测过敏原特异性 IgE 的临床性能。

<table>
	<tbody>
		<tr>
			<td>Agnitio BioIC Analyzer</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>BA-G2000</td>
			<td></td>
		</tr>
		<tr>
			<td>BioIC Allergen specific-IgE Detection Kit</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>DR17A12</td>
			<td></td>
		</tr>
		<tr>
			<td>BioIC Cartrideg Placement plate</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>T20SET</td>
			<td></td>
		</tr>
		<tr>
			<td>BioIC Reagent Dispenser</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>DS-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Image two-dimensional barcode machine</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>NLS-HR200</td>
			<td></td>
		</tr>
		<tr>
			<td>Software Package, LabIT</td>
			<td>Agnitio Science &#38; Technology(Taiwan, China)</td>
			<td>Version 2.4.12</td>
			<td></td>
		</tr>
		<tr>
			<td>VORTEX-5 Vortex Mixer</td>
			<td>Haimen Kylinbell Lab Lastruments Co., Ltd.</td>
			<td>VORTEX-5</td>
			<td></td>
		</tr>
	</tbody>
</table>

application of biochip microfluidic technology to detect serum allergen-specific immunoglobulin e (sige)

过敏性疾病在成人和儿童中都很常见。病因过敏原的鉴定在疾病管理和预防中具有重要意义。然而, 中国大陆, 特别是初级保健医院, 缺乏具有较高性价比的特定免疫球蛋白 e (IgE) 测量系统。本文介绍了利用微流化物盒化学发光系统检测血清中过敏原特异性 IgE 的原理和操作步骤。并将结果与免疫 Cap (系统 1)、工业标准和该系统的重现性进行了比较, 以检测对常见过敏原敏感的患者。结果表明, 与免疫 Cap (系统 1) 相比, 生物 Ic 系统 (系统 2) 在检测血清特异性 IgE (Ige) 时具有较好的精度和灵敏度, 但成本要低得多。它可以作为中国大陆初级保健医院系统1的一个很好的替代方案, 因为这些医院的经济承受能力较低。

19种常见过敏原的阳性率 293 sera 的结果如图 3所示。在所有吸入过敏原中, d. farinae的阳性率最高 (80.89, 271-293), 其次是d.翼状鼻窦炎(78.84, 231/293)。在食物过敏原中, 螃蟹的阳性率最高 (20.48, 60/293), 其次是 13.65 (40/293)。吸入性过敏原的总阳性率高于食物过敏原。
微流?...

Watch this Scientific Journal Video about Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE at JoVE.com

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

本文提出了一种基于微流控剂的化学发光系统检测血清特异性免疫球蛋白 E 的方法, 并评价其在过敏诊断中的应用价值。

生物芯片微流控技术在血清过敏源特异性免疫球蛋白 E (sIgE) 检测中的应用

Allergic disease is common in both adults and children. Identification of the causative allergens is significant in disease management and prevention. ...

生物芯片微流体技术系统是一个基于微流体的分部，它使用化学发光原理对血清规格IGE进行分析。与其他检测方法相比，BioIC系统具有成本低、血清检测血清ICE要求少等优点。这种技术的影响倾向于诊断过敏性疾病，如鼻炎和哮喘，因为许多这些患者是敏感于常见的可检测过敏原。虽然这种方法可以提供呼吸道过敏性疾病的见解，它也可以适用于其他情况，如食物和皮肤过敏。由于此技术更易于使用，并且具有降低操作员培训的方法，因此系统新人员可以学会快速使用它。这一问题生动地演示至关重要，因为确定测试是否成功，并且需要熟练的手动操作。要测试血清样本的血清免疫球蛋白E抗体对整个过敏原，随机选择三个样本与至少900微升血清，并采取芯片从套件和编号。然后，打开计算机和分析仪电源。在计算机上启动实验室 IT 程序。如果弹出暗框警告窗口，请单击"确定"以运行泄漏测试，然后单击中心徽标以进入操作界面。检查反应温度和电荷耦合装置或CCD温度，反应温度应在10分钟内上升到37°或零下1摄氏度，CCD温度应降至零下15°C或1°C。当 CCD 温度降至零下 15°C 或零下一摄氏度时，请确认仪器内没有其他物品，然后关上门。然后单击工具、系统测试、泄漏测试。测试完成后，将弹出报告窗口。接下来，将620微升的洗涤缓冲液、120微升的阻尼缓冲液、60微升的联结A和B、60微升基板A和B、100微升的血清样品装入微流体盒上的相应试剂罐中。单击墨盒 ID 后，使用条形码扫描仪扫描墨盒的序列号并输入样本 ID。在分析结束时，将结果导出到适当的统计软件程序中，然后从分析仪中取出墨盒。用 75% 的酒精轻轻擦拭分析仪的内部加热铁和电磁铁，并关闭实验室 IT 窗口。温度监测窗口将弹出，当 CCD 预热至 5 摄氏度的保护模式时，将自动关闭，此时可以安全地关闭分析仪和计算机。微流体系统产生可重复的结果，如这些数据证明猫皮屑，狗皮屑和蟑螂经过九轮测试。如所观察，所有数据都在两个标准差的范围内，这与允许的最大临床误差一致。正如过敏原的正率所预期的那样，所有吸入剂过敏原的评估结果都优于食物过敏原。由于曲线值下的面积显示范围为 0.613 到 0.984，并且三个吸入剂过敏原的曲线值下的区域大于 0.950，因此可以得出结论，系统二具有高精度，参照系统一。两个系统的一致性分析显示，三种吸入剂的卡帕值都大于食物过敏原的 kappa 值，通常低于 0.4。在这些散射图中，这些散射图采用沿 x 轴的系统二个结果构建，系统一个结果沿 y 轴构建，可以观察到两个系统对六种不同过敏原的血清免疫球蛋白 E 浓度的分布。在开始检测之前，始终检查反应和 CCD 温度，因为它们与测试的准确性相关。按照此过程，可以执行其他方法，如组件解析诊断，以回答有关过敏原交叉标准化和共向化的其他问题。微流体芯片技术已成功应用于过敏诊断领域，以解决血清不足、医疗费用高等问题。执行此程序时，应始终佩戴适当的个人防护设备和其他预防措施。

Research

JoVE Journal

Medicine

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

可视化和肝脏微循环血流量和耗氧量分析：急性肝炎模型中的应用

There is a considerable discrepancy between oxygen supply and demand in the liver because hepatic oxygen consumption is relatively high but about 70% of ...

科研

医学

Using Continuous Data Tracking Technology to Study Exercise Adherence in Pulmonary Rehabilitation

使用连续数据跟踪技术在肺康复研究锻炼坚持性

Pulmonary rehabilitation (PR) is an important component in the management of respiratory diseases. The effectiveness of PR is dependent upon adherence to ...

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

高通量流式细胞仪检测细胞为基础的试验检测抗体，以N-甲基-D-天冬氨酸受体或多巴胺2受体在人血清

Over the recent years, antibodies against surface and conformational proteins involved in neurotransmission have been detected in autoimmune CNS diseases ...

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

适应人类Videofluoroscopic燕子研究的方法来检测和检定吞咽困难的小鼠疾病模型

This study adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating ...

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

VDJ-SEQ：重排免疫球蛋白重链基因的深度测序分析，以揭示的B细胞淋巴瘤克隆演变模式

Understanding tumor clonality is critical to understanding the mechanisms involved in tumorigenesis and disease progression. In addition, understanding ...

Application of End-to-end Anastomosis in Robotic Central Pancreatectomy

端到端吻合技术在机器人中心胰腺中的应用

Central pancreatectomy is carried out for the treatment of benign or low-malignant potential tumors located in the pancreatic neck or proximal part of ...

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

质子治疗在实体肿瘤恶性肿瘤选择中的临床应用

Radiation therapy is a frequently used modality for the treatment of solid cancers. Although the mechanisms of cell kill are similar for all forms of ...

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

免疫球蛋白 G N-甘油分析通过超高性能液色谱分析

Glycomics is a new subspecialty in omics system research that offers significant potential in discovering next-generation biomarkers for disease ...

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice

分析长期心电图记录以检测小鼠心律失常

Arrhythmias are common, affecting millions of patients worldwide. Current treatment strategies are associated with significant side effects and remain ...

Nerve Ultrasound Protocol to Detect Dysimmune Neuropathies

检测免疫异常神经病的神经超声方案

Nerve ultrasound is increasingly used in the differential diagnosis of polyneuropathy as a complementary tool to nerve conduction studies. Morphological ...