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DOI :
10.3791/59104-v
March 24th, 2019
Chapters
0:04
Title
1:00
Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
3:02
Cell Lysis and Protein Transfer
5:28
Histone Post-translational Modification Detection
6:52
Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
8:03
Conclusion
该协议概述了实验程序, 以描述全基因组的变化, 在与 ALS 和帕金森病相关的蛋白质过度表达的情况下, 组蛋白翻译后修饰 (PTM) 的水平发生。酿酒酵母模型。SDS-PAGE 分离后, 通过西方印迹, 通过修正特异性抗体检测单个组蛋白 PTM 水平。
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