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DOI :
10.3791/59124-v
March 5th, 2019
Chapters
0:04
Title
0:52
Isolation of Spleens from Mice
1:27
Generation of Single Cell Suspensions from Spleens
2:39
Isolation of CD11c+ DCs from Splenic Single Cell Suspension
3:41
Magnetic Separation of CD11c+ DCs
4:51
Adoptive Transfer of High Salt Treated CD11c+ DCs
6:56
Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
8:52
Conclusion
여기, 선물이 자기 세포 분류와 순진한 쥐로 후속 입양 전송 murine spleens에서 수지상 세포를 분리 하는 프로토콜. 높은 소금 활성화 수지상 세포의 모델 입양 전송 및 cytometry의 단계별 절차를 설명 하기 위해 선택 되었다.
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