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Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

13.4K Views

08:32 min

February 12th, 2019

February 12th, 2019

13,377 Views

0:04

Title

1:05

Conditioned Medium Collection and Processing

2:45

Nanoparticle Tracking Analysis (NTA) Quantification

3:27

Sample Preparation

4:17

Sample Acquisition and Analysis

6:32

Results: Representative Flow Cytometric Analyses of Extracellular Vesicles from Cell-conditioned Medium

7:58

Conclusion

Transcript

Extracellular Vesicles are increasingly being used as a diagnostic biomarker and potential therapeutic tool in many field. Flow Cytometric is the most straight forward analytic method for characterizing by suit faced markers this protocol can be u

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The protocol describes a reproducible method designed for use with cell culture supernatants to detect surface epitopes on small extracellular vesicles (EV). It utilizes specific EV immunoprecipitation using beads coupled with antibodies that recognize surface antigen CD9, CD63, and CD81. The method is optimized for downstream flow cytometry analysis.

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