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DOI :
10.3791/59133-v
•
7:55 min
March 7th, 2019
Chapters
0:04
Title
0:32
Plate Reader Assay
3:13
Selection of Suppressor Colonies and Confirmation of Phenotype
5:27
Results: Slow-growing Mutants Show Phenotypic Recovery and Genetic Confirmation of Identified Suppressor Mutations Verifies the Heritability of the Recovery Phenotype
6:56
Conclusion
酵母分裂簡便なサプレッサー スクリーンのプロトコルを提案します。この方法は、効率的な変異原無料、頻繁に単一の genomic 位置で起こる突然変異の選択です。 プロトコルは、突然変異や薬物によって引き起こされる液体文化で成長欠陥を緩和抑制を分離することに適しています。
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