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DOI :
10.3791/59249-v
March 26th, 2019
Chapters
0:04
Title
0:53
Immunization of C57BL/6 Mice
2:04
Scoring of EAE Mice
3:08
In Vivo Permeability Assay
4:25
Dissection and Freezing of Brains
6:15
In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
8:21
Results: Course of EAE and In Vivo Permeability
9:42
Conclusion
生体内で実験的自己免疫性脳脊髄炎時単位の障害を調査するためのプロトコルを紹介します。具体的にはグリア limitans にわたってゼラチナーゼ活性の白血球の遊走に関与する血液-脳関門の透過性を確認する方法に取り組みます。
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