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DOI :
10.3791/59267-v
•
8:52 min
February 17th, 2019
Chapters
0:04
Title
0:49
Micro-Injection Pipette Preparation
1:53
Host Embryo Preparation
3:34
Donor Tissue Isolation
4:44
Donor Tissue Digestion
5:50
In Vivo Injection
7:37
Results: Representative Donor Myocyte-Implanted Embryonic Recipient Heart Imaging
8:14
Conclusion
この方法では胚の心臓組織は外科的 microdissected、解離、蛍光標識、そして宿主胚性組織に注入です。これは個人または組織レベル発達組織下異所性血行力学的条件、および/またはパラクリン/juxtacrine 環境の変化を研究するためのプラットフォームを提供します。
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