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DOI :
10.3791/59288-v
•
12:58 min
March 26th, 2019
Chapters
0:04
Title
2:10
Cell Treatments and Laser Setup
3:44
Image Acquisition Setup and Collection
5:54
Data Analysis
8:08
Data Analysis in a Spreadsheet Program
10:16
Results: Fluorescence Recovery After Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
11:20
Conclusion
我们描述了一种全面和实用的方法, 荧光恢复后的光漂白实验与活细胞。虽然该协议被用来测量黄色荧光蛋白标记 p62 在类似侵犯诱导结构中的流动性, 但它可以应用于各种显微镜系统和荧光蛋白。
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