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DOI :
10.3791/59294-v
•
11:25 min
May 19th, 2019
Chapters
0:04
Title
1:38
Supercomplex Extraction
2:36
Gradient Gel Casting and Electrophoresis
6:50
In-gel Activity for Complexes I, II, IV and V
8:24
Results
10:01
Conclusion
在这里, 我们提出了一个协议, 以提取, 解决和识别线粒体超级复合物, 最大限度地减少接触洗涤剂和库马西蓝。该协议提供了分辨率和酶活性保存之间的最佳平衡, 同时最大限度地降低了失去不稳定的蛋白质-蛋白质相互作用的风险。
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