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DOI :
10.3791/59294-v
•
11:25 min
May 19th, 2019
Chapters
0:04
Title
1:38
Supercomplex Extraction
2:36
Gradient Gel Casting and Electrophoresis
6:50
In-gel Activity for Complexes I, II, IV and V
8:24
Results
10:01
Conclusion
ここでは、洗剤とクマシーブルーへの暴露を最小限に抑えるミトコンドリア supercomplexes を抽出、解決、識別するためのプロトコルを紹介します。このプロトコルは、分解能と酵素活性の維持との間の最適なバランスを提供しながら、不安定なタンパク質間相互作用を失うリスクを最小限に抑えます。
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