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DOI :
10.3791/59308-v
June 25th, 2019
Chapters
0:04
Title
1:13
Tissue Cutting
1:45
Blocking of Nonspecific Binding Sites and Tissue Permeabilization
2:44
Incubation with Antibodies
3:18
Tissue Mounting
3:56
Controls
4:26
Image Acquisition
5:31
Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
7:18
Conclusion
这里介绍的是免疫组织化学表征和定位的orexin肽,orexin受体,和内分泌大麻素受体在肠道和大脑中正常和饮食诱发肥胖(DIO)成年斑马鱼模型使用免疫渗透酶和双免疫荧光法。
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