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DOI :
10.3791/59308-v
June 25th, 2019
Chapters
0:04
Title
1:13
Tissue Cutting
1:45
Blocking of Nonspecific Binding Sites and Tissue Permeabilization
2:44
Incubation with Antibodies
3:18
Tissue Mounting
3:56
Controls
4:26
Image Acquisition
5:31
Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
7:18
Conclusion
ここでは、オレキシンペプチド、オレキシン受容体、および正常および食事誘発肥満(DIO)成人ゼブラフィッシュモデルの腸および脳における内因性カンナビノイド受容体の免疫組織化学的特徴付けおよび局在化のためのプロトコルを示す。免疫ペリキシダーゼおよび二重免疫蛍光法。
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