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DOI :
10.3791/59321-v
May 1st, 2019
Chapters
0:04
Title
0:54
Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation
2:19
Antibiotic Selection
3:18
Motor Neuron Differentiation
5:12
Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis
7:01
Conclusion
이 프로토콜은 유도 만능 줄기 세포를 유도성 piggyBac 벡터에서 전사 인자의 발현에 의해 척추 또는 두개골 정체성을 가진 모터 뉴런으로 신속 하 고 효율적으로 전환 할 수 있습니다.
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