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DOI :
10.3791/59339-v
•
8:19 min
April 22nd, 2019
Chapters
0:04
Title
1:12
Preparation of Keratinocyte Culture Medium and the Fibril Form of Type I Collagen
2:53
Preparation of the Non-fibrous Form of Type I Collagen
3:50
Culture of FEPE1L-8 Cells
5:20
Estimation of the Number of Viable Cells
6:20
Results: Evaluation of Keratinocyte Proliferation
7:01
Conclusion
在这里, 我们提出了一个协议, 用于利用 I 型胶原蛋白制备两种不同形式的培养基板。根据胶原蛋白的处理方式, 胶原蛋白分子要么保持二维, 非纤维状的形式, 要么重新组装成三维的, 纤维形式。I 型胶原蛋白上的细胞增殖受到纤维形成的极大影响。
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