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DOI :
10.3791/59350-v
•
10:18 min
May 28th, 2019
Chapters
0:04
Title
1:22
Single CRISPR RNA (crRNA) Array Expression Cassette
2:10
S. cerevisiae Transformation
5:02
Second Transformation
6:42
Genome Editing Efficiency Evaluation and Yeast Pixel Art Creation
8:30
Results: Transformation Verification and Yeast Pixel Art Creation
9:39
Conclusion
CRISPR/Cas12aシステムは単一のcrRNA配列と結合して複数の遺伝子座のS.セレビシエのゲノムの有効な多重編集を同時に可能にする。これは、その後、酵母ピクセル技術を作成するために使用される酵母株を生産するカロテノイドを構築することによって実証されます。
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